Cell Cycle Antibodies 1

Anti-ZC3HC1 Antibody (CAB6331)

(No reviews yet) Write a Review
SKU:
CAB6331
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ZC3HC1 Antibody
Antibody SKU:CAB6331
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZC3HC1 (NP_057562.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZC3HC1 (NP_057562.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAP CEGQ AFAV GVEK NWGA VVRS PEGT PQKI RQLI DEGI APEE GGVD AKDT SATS QSVN GSPQ AEQP SLES TSKE AFFS RVET FSSL KWAG KPFE LSPL VCAK YGWV TVEC DMLK CSSC QAFL CASL QPAF DFDR YKQR CAEL KKAL CTAH EKFC FWPD SPSP DRFG MLPL DEPA ILVS EFLD RFQS LCHL DLQL PSLR PEDL KTMC LTED KISL LLHL LEDE LDHR TDER KTTI KLGS DIQV HVTA CILS VCGW ACSS
Gene ID:51530
Uniprot:Q86WB0
Cellular Location:Nucleus
Calculated MW:47kDa/53kDa/55kDa
Observed MW:55kDa
Synonyms:ZC3HC1, HSPC216, NIPA
Background:This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis.
UniProt Protein Function:NIPA: a nuclear protein that interacts with anaplastic lymphoma kinase (ALK). An essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1. Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting cyclin B1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of cyclin B1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. Interacts with the NPM-ALK fusion protein in a tyrosine phosphorylation-dependent manner. Three splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 7q32.2

Cellular Component: nuclear membrane; nucleus

Molecular Function:protein binding; protein kinase binding; zinc ion binding

Biological Process: cell division; mitosis; protein ubiquitination

NCBI Summary:This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis. [provided by RefSeq, Aug 2013]
UniProt Code:Q86WB0
NCBI GenInfo Identifier:73921220
NCBI Gene ID:51530
NCBI Accession:Q86WB0.1
UniProt Secondary Accession:Q86WB0,Q75MF3, Q75MF4, Q8N330, Q96F75, Q9HA34, Q9NVX4 Q9P0R0, A6NH66,
UniProt Related Accession:Q86WB0
Molecular Weight:47,771 Da
NCBI Full Name:Nuclear-interacting partner of ALK
NCBI Synonym Full Names:zinc finger C3HC-type containing 1
NCBI Official Symbol:ZC3HC1  
NCBI Official Synonym Symbols:NIPA; HSPC216  
NCBI Protein Information:nuclear-interacting partner of ALK
UniProt Protein Name:Nuclear-interacting partner of ALK
UniProt Synonym Protein Names:Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1
Protein Family:NIPA-like protein
UniProt Gene Name:ZC3HC1  
UniProt Entry Name:NIPA_HUMAN
Anti-ZC3HC1 Antibody (CAB6331)Western blot analysis of extracts of HeLa cells, using ZC3HC1 antibody (CAB6331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products