Immunology Antibodies 2
Anti-ZAP70 Antibody (CAB2195)
- SKU:
- CAB2195
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-ZAP70 Antibody |
| Antibody SKU: | CAB2195 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ZAP70 (NP_001070.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ZAP70 (NP_001070.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPDP AAHL PFFY GSIS RAEA EEHL KLAG MADG LFLL RQCL RSLG GYVL SLVH DVRF HHFP IERQ LNGT YAIA GGKA HCGP AELC EFYS RDPD GLPC NLRK PCNR PSGL EPQP GVFD CLRD AMVR DYVR QTWK LEGE ALEQ AIIS QAPQ VEKL IATT AHER MPWY HSSL TREE AERK LYSG AQTD GKFL LRPR KEQG TYAL SLIY GKTV YHYL ISQD KAGK YCIP EGTK FDTL WQLV EYLK LKAD GLIY CLKE ACPN SSAS NASG AAAP TLPA HPST LTHP QRRI DTLN SDGY TPEP ARIT |
| Gene ID: | 7535 |
| Uniprot: | P43403 |
| Cellular Location: | Cell membrane, Cytoplasm, Peripheral membrane protein |
| Calculated MW: | 35kDa/55kDa/69kDa |
| Observed MW: | 70KDa |
| Synonyms: | ZAP70, ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70 |
| Background: | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. |
| UniProt Protein Function: | ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Syk family Chromosomal Location of Human Ortholog: 2q12 Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; immunological synapse; intercellular junction; lipid raft; plasma membrane; T cell receptor complex Molecular Function:ATP binding; non-membrane spanning protein tyrosine kinase activity; phosphotyrosine binding; protein binding; protein-tyrosine kinase activity; receptor binding Biological Process: adaptive immune response; B cell activation; B cell receptor signaling pathway; beta selection; immune response; innate immune response; macrophage activation during immune response; negative thymic T cell selection; neutrophil activation during immune response; peptidyl-tyrosine phosphorylation; positive regulation of alpha-beta T cell differentiation; positive regulation of alpha-beta T cell proliferation; positive regulation of B cell differentiation; positive regulation of calcium-mediated signaling; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; positive regulation of T cell differentiation; positive thymic T cell selection; protein amino acid phosphorylation; T cell activation; T cell differentiation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway Disease: Selective T-cell Defect |
| NCBI Summary: | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P43403 |
| NCBI GenInfo Identifier: | 1177044 |
| NCBI Gene ID: | 7535 |
| NCBI Accession: | P43403.1 |
| UniProt Secondary Accession: | P43403,Q6PIA4, Q8IXD6, Q9UBS6, A6NFP4, |
| UniProt Related Accession: | P43403 |
| Molecular Weight: | 55,873 Da |
| NCBI Full Name: | Tyrosine-protein kinase ZAP-70 |
| NCBI Synonym Full Names: | zeta chain of T cell receptor associated protein kinase 70kDa |
| NCBI Official Symbol: | ZAP70 |
| NCBI Official Synonym Symbols: | SRK; STD; TZK; STCD; ZAP-70 |
| NCBI Protein Information: | tyrosine-protein kinase ZAP-70 |
| UniProt Protein Name: | Tyrosine-protein kinase ZAP-70 |
| UniProt Synonym Protein Names: | 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase |
| Protein Family: | Tyrosine-protein kinase |
| UniProt Gene Name: | ZAP70 |
| UniProt Entry Name: | ZAP70_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ZAP70 antibody (CAB2195) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded human amygdalitis using ZAP70 antibody (CAB2195) at dilution of 1:100 (40x lens). |
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