Cell Biology Antibodies 8

Anti-YWHAQ Antibody (CAB2563)

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SKU:
CAB2563
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-YWHAQ Antibody
Antibody SKU:CAB2563
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human YWHAQ (NP_006817.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, BT-474, HeLa, Jurkat, Mouse liver, Mouse kidney, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human YWHAQ (NP_006817.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEKT ELIQ KAKL AEQA ERYD DMAT CMKA VTEQ GAEL SNEE RNLL SVAY KNVV GGRR SAWR VISS IEQK TDTS DKKL QLIK DYRE KVES ELRS ICTT VLEL LDKY LIAN ATNP ESKV FYLK MKGD YFRY LAEV ACGD DRKQ TIDN SQGA YQEA FDIS KKEM QPTH PIRL GLAL NFSV FYYE ILNN PELA CTLA KTAF DEAI AELD TLNE DSYK DSTL IMQL LRDN LTLW TSDS AGEE CDAA EGAE N
Gene ID:10971
Uniprot:P27348
Cellular Location:Cytoplasm
Calculated MW:27kDa
Observed MW:32kDa
Synonyms:YWHAQ, 14-3-3, 1C5, HS1
Background:This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
UniProt Protein Function:14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane; mitochondrion; protein complex

Molecular Function:protein binding; protein C-terminus binding; protein domain specific binding; protein N-terminus binding

Biological Process: apoptosis; gene expression; negative regulation of transcription, DNA-dependent; programmed cell death; small GTPase mediated signal transduction; substantia nigra development; transcription initiation from RNA polymerase II promoter

NCBI Summary:This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]
UniProt Code:P27348
NCBI GenInfo Identifier:112690
NCBI Gene ID:10971
NCBI Accession:P27348.1
UniProt Secondary Accession:P27348,Q567U5, Q5TZU8, Q9UP48, D6W4Z5,
UniProt Related Accession:P27348
Molecular Weight:
NCBI Full Name:14-3-3 protein theta
NCBI Synonym Full Names:tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
NCBI Official Symbol:YWHAQ  
NCBI Official Synonym Symbols:1C5; HS1; 14-3-3  
NCBI Protein Information:14-3-3 protein theta
UniProt Protein Name:14-3-3 protein theta
UniProt Synonym Protein Names:14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1
Protein Family:14-3-3 protein
UniProt Gene Name:YWHAQ  
UniProt Entry Name:1433T_HUMAN
Anti-YWHAQ Antibody (CAB2563)Western blot analysis of extracts of various cell lines, using YWHAQ antibody (CAB2563) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-YWHAQ Antibody (CAB2563)Immunofluorescence analysis of HeLa cells using YWHAQ antibody (CAB2563). Blue: DAPI for nuclear staining.
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