Immunology Antibodies 1
Anti-YWHAE Antibody (CAB1058)
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- SKU:
- CAB1058
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-YWHAE Antibody |
| Antibody SKU: | CAB1058 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human YWHAE (NP_006752.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, U-251MG, Mouse brain, Mouse kidney, Mouse lung, Rat brain, Rat kidney, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human YWHAE (NP_006752.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDDR EDLV YQAK LAEQ AERY DEMV ESMK KVAG MDVE LTVE ERNL LSVA YKNV IGAR RASW RIIS SIEQ KEEN KGGE DKLK MIRE YRQM VETE LKLI CCDI LDVL DKHL IPAA NTGE SKVF YYKM KGDY HRYL AEFA TGND RKEA AENS LVAY KAAS DIAM TELP PTHP IRLG LALN FSVF YYEI LNSP DRAC RLAK AAFD DAIA ELDT LSEE SYKD STLI MQLL RDNL TLWT SDMQ GDGE EQNK EALQ DVED ENQ |
| Gene ID: | 7531 |
| Uniprot: | P62258 |
| Cellular Location: | Cytoplasm, Melanosome |
| Calculated MW: | 26kDa/29kDa |
| Observed MW: | 29-32Kda |
| Synonyms: | YWHAE, 14-3-3E, HEL2, KCIP-1, MDCR, MDS |
| Background: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. |
| UniProt Protein Function: | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). |
| NCBI Summary: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008] |
| UniProt Code: | P62258 |
| NCBI GenInfo Identifier: | 51702210 |
| NCBI Gene ID: | 7531 |
| NCBI Accession: | P62258.1 |
| UniProt Secondary Accession: | P62258,P29360, P42655, Q4VJB6, Q53XZ5, Q63631, Q7M4R4 B3KY71, D3DTH5, |
| UniProt Related Accession: | P62258 |
| Molecular Weight: | 26,504 Da |
| NCBI Full Name: | 14-3-3 protein epsilon |
| NCBI Synonym Full Names: | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon |
| NCBI Official Symbol: | YWHAE |
| NCBI Official Synonym Symbols: | MDS; HEL2; MDCR; KCIP-1; 14-3-3E |
| NCBI Protein Information: | 14-3-3 protein epsilon |
| UniProt Protein Name: | 14-3-3 protein epsilon |
| Protein Family: | 14-3-3 protein |
| UniProt Gene Name: | YWHAE |
 | Western blot analysis of extracts of various cell lines, using YWHAE antibody (CAB1058) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of H9C2 cells using YWHAE antibody (CAB1058) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using YWHAE antibody (CAB1058) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using YWHAE antibody (CAB1058) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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