Cell Biology Antibodies 12
Anti-YTHDF3 Antibody (CAB8395)
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- SKU:
- CAB8395
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-YTHDF3 Antibody |
| Antibody SKU: | CAB8395 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human YTHDF3 (NP_689971.4). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-1080, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human YTHDF3 (NP_689971.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSAT SVDQ RPKG QGNK VSVQ NGSI HQKD AVND DDFE PYLS SQTN QSNS YPPM SDPY MPSY YAPS IGFP YSLG EAAW STAG DQPM PYLT TYGQ MSNG EHHY IPDG VFSQ PGAL GNTP PFLG QHGF NFFP GNAD FSTW GTSG SQGQ STQS SAYS SSYG YPPS SLGR AITD GQAG FGND TLSK VPGI SSIE QGMT GLKI GGDL TAAV TKTV GTAL SSSG MTSI ATNS VPPV SSAA PKPT SWAA IARK PAKP QPKL KPKG NVGI GGSA VPPP PIKH NMN |
| Gene ID: | 253943 |
| Uniprot: | Q7Z739 |
| Cellular Location: | |
| Calculated MW: | 63kDa |
| Observed MW: | 64kDa |
| Synonyms: | YTHDF3 |
| Background: | This gene encodes a member of the YTH (YT521-B homology) domain protein family. The YTH domain is common in eukaryotes, is often found in the middle of the protein sequence, and may function in binding to RNA. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | YTHDF3: Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability |
| UniProt Protein Details: | Protein type:RNA-binding Chromosomal Location of Human Ortholog: 8q12.3 Molecular Function:protein binding |
| NCBI Summary: | This gene encodes a member of the YTH (YT521-B homology) domain protein family. The YTH domain is common in eukaryotes, is often found in the middle of the protein sequence, and may function in binding to RNA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013] |
| UniProt Code: | Q7Z739 |
| NCBI GenInfo Identifier: | 74738853 |
| NCBI Gene ID: | 253943 |
| NCBI Accession: | Q7Z739.1 |
| UniProt Secondary Accession: | Q7Z739,Q63Z37, Q659A3, B3KXL4, |
| UniProt Related Accession: | Q7Z739 |
| Molecular Weight: | 63,861 Da |
| NCBI Full Name: | YTH domain-containing family protein 3 |
| NCBI Synonym Full Names: | YTH N6-methyladenosine RNA binding protein 3 |
| NCBI Official Symbol: | YTHDF3 |
| NCBI Protein Information: | YTH domain-containing family protein 3 |
| UniProt Protein Name: | YTH domain-containing family protein 3 |
| Protein Family: | YTH domain-containing family protein |
| UniProt Gene Name: | YTHDF3 |
| UniProt Entry Name: | YTHD3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using YTHDF3 Antibody (CAB8395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry of paraffin-embedded human esophagus using YTHDF3 antibody (CAB8395) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using YTHDF3 antibody (CAB8395) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse stomach using YTHDF3 antibody (CAB8395) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using YTHDF3 antibody (CAB8395) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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