Signal Transduction Antibodies 1

Anti-YOD1 Antibody (CAB13270)

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SKU:
CAB13270
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-YOD1 Antibody
Antibody SKU:CAB13270
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human YOD1 (NP_061036.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human YOD1 (NP_061036.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MFGP AKGR HFGV HPAP GFPG GVSQ QAAG TKAG PAGA WPVG SRTD TMWR LRCK AKDG THVL QGLS SRTR VREL QGQI AAIT GIAP GGQR ILVG YPPE CLDL SNGD TILE DLPI QSGD MLII EEDQ TRPR SSPA FTKR GASS YVRE TLPV LTRT VVPA DNSC LFTS VYYV VEGG VLNP ACAP EMRR LIAQ IVAS DPDF YSEA ILGK TNQE YCDW IKRD DTWG GAIE ISIL SKFY QCEI CVVD TQTV RIDR FGED AGYT KRVL LIYD GIHY DPLQ RNFP DPDT PPLT IFSS NDDI VLVQ ALEL ADEA RRRR QFTD VNRF TLRC MVCQ KGLT GQAE AREH AKET GHTN FGEV
Gene ID:55432
Uniprot:Q5VVQ6
Cellular Location:
Calculated MW:33kDa/38kDa
Observed MW:41kDa
Synonyms:YOD1, DUBA8, OTUD2, PRO0907
Background:Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. The protein encoded by this gene belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:YOD1: Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di- ubiquitin. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Lipid Metabolism - unsaturated fatty acid biosynthesis; Protease; EC 3.4.19.12; Ubiquitin-specific protease; C2H2-type zinc finger protein; Secondary Metabolites Metabolism - limonene and pinene degradation

Chromosomal Location of Human Ortholog: 1q32.2

Molecular Function:protein binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity

Biological Process: ER-associated protein catabolic process; unfolded protein response

NCBI Summary:Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. The protein encoded by this gene belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
UniProt Code:Q5VVQ6
NCBI GenInfo Identifier:74747276
NCBI Gene ID:55432
NCBI Accession:Q5VVQ6.1
UniProt Secondary Accession:Q5VVQ6,Q5VVQ5, Q6ZRS6, Q86T63, Q9P1L8, B2RNX3,
UniProt Related Accession:Q5VVQ6
Molecular Weight:33,979 Da
NCBI Full Name:Ubiquitin thioesterase OTU1
NCBI Synonym Full Names:YOD1 deubiquitinase
NCBI Official Symbol:YOD1  
NCBI Official Synonym Symbols:DUBA8; OTUD2; PRO0907  
NCBI Protein Information:ubiquitin thioesterase OTU1
UniProt Protein Name:Ubiquitin thioesterase OTU1
UniProt Synonym Protein Names:DUBA-8; HIV-1-induced protease 7; HIN-7; HsHIN7; OTU domain-containing protein 2
UniProt Gene Name:YOD1  
UniProt Entry Name:OTU1_HUMAN
Anti-YOD1 Antibody (CAB13270)Western blot analysis of extracts of mouse brain, using YOD1 antibody (CAB13270) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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