Metabolism Antibodies 2

Anti-YARS Antibody (CAB6791)

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SKU:
CAB6791
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-YARS Antibody
Antibody SKU:CAB6791
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human YARS (NP_003671.1).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, SKOV3, Mouse brain, Mouse skeletal muscle, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human YARS (NP_003671.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGDA PSPE EKLH LITR NLQE VLGE EKLK EILK EREL KIYW GTAT TGKP HVAY FVPM SKIA DFLK AGCE VTIL FADL HAYL DNMK APWE LLEL RVSY YENV IKAM LESI GVPL EKLK FIKG TDYQ LSKE YTLD VYRL SSVV TQHD SKKA GAEV VKQV EHPL LSGL LYPG LQAL DEEY LKVD AQFG GIDQ RKIF TFAE KYLP ALGY SKRV HLMN PMVP GLTG SKMS SSEE ESKI DLLD RKED VKKK LKKA FCEP GNVE NNGV LSFI KHVL FPLK SEFV ILRD EKWG GNKT YTAY VDLE KDFA
Gene ID:8565
Uniprot:P54577
Cellular Location:Cytoplasm
Calculated MW:59kDa
Observed MW:59kDa
Synonyms:YARS, CMTDIC, TYRRS, YRS, YTS
Background:Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
UniProt Protein Function:YARS: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC). CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. Belongs to the class-I aminoacyl-tRNA synthetase family.
UniProt Protein Details:

Protein type:EC 6.1.1.1; Ligase

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: cytoplasm; cytosol; extracellular space; nucleus

Molecular Function:interleukin-8 receptor binding; protein binding; tyrosine-tRNA ligase activity; valine-tRNA ligase activity

Biological Process: apoptosis; tRNA aminoacylation for protein translation; valyl-tRNA aminoacylation

Disease: Charcot-marie-tooth Disease, Dominant Intermediate C

NCBI Summary:Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]
UniProt Code:P54577
NCBI GenInfo Identifier:13638438
NCBI Gene ID:8565
NCBI Accession:P54577.4
UniProt Secondary Accession:P54577,O43276, Q53EN1, B3KWK4, D3DPQ4,
UniProt Related Accession:P54577
Molecular Weight:59,143 Da
NCBI Full Name:Tyrosine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:tyrosyl-tRNA synthetase
NCBI Official Symbol:YARS  
NCBI Official Synonym Symbols:YRS; YTS; TYRRS; CMTDIC  
NCBI Protein Information:tyrosine--tRNA ligase, cytoplasmic
UniProt Protein Name:Tyrosine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Tyrosyl-tRNA synthetase; TyrRS
Protein Family:Tyrosine--tRNA ligase
UniProt Gene Name:YARS  
UniProt Entry Name:SYYC_HUMAN
Anti-YARS Antibody (CAB6791)Western blot analysis of extracts of various cell lines, using YARS antibody (CAB6791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-YARS Antibody (CAB6791)Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug YARS antibody (CAB6791). Western blot was performed from the immunoprecipitate using YARS antibody (CAB6791) at a dilition of 1:1000.
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