Antibody Name:	Anti-XRCC1 Antibody
Antibody SKU:	CAB0442
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human XRCC1 (NP_006288.2).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human XRCC1 (NP_006288.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPEI RLRH VVSC SSQD STHC AENL LKAD TYRK WRAA KAGE KTIS VVLQ LEKE EQIH SVDI GNDG SAFV EVLV GSSA GGAG EQDY EVLL VTSS FMSP SESR SGSN PNRV RMFG PDKL VRAA AEKR WDRV KIVC SQPY SKDS PFGL SFVR FHSP PDKD EAEA PSQK VTVT KLGQ FRVK EEDE SANS LRPG ALFF SRIN KTSP VTAS DPAG PSYA AATL QASS AASS ASPV SRAI GSTS KPQE SPKG KRKL DLNQ EEKK TPSK PPAQ LSPS VPKR PKLP APTR TPAT APVP ARAQ GAVT GKPR GEGT EPRR PRAG PEEL GKIL
Gene ID:	7515
Uniprot:	P18887
Cellular Location:	Nucleus
Calculated MW:	69kDa
Observed MW:	69kDa

Synonyms:

XRCC1, RCC

Background:

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

UniProt Protein Function:	XRCC1: Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.
UniProt Protein Details:	Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; enzyme binding; damaged DNA binding Biological Process: single strand break repair; base-excision repair; DNA repair
NCBI Summary:	The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
UniProt Code:	P18887
NCBI GenInfo Identifier:	317373290
NCBI Gene ID:	7515
NCBI Accession:	P18887.2
UniProt Secondary Accession:	P18887,Q6IBS4, Q9HCB1,
UniProt Related Accession:	P18887
Molecular Weight:	69,477 Da
NCBI Full Name:	DNA repair protein XRCC1
NCBI Synonym Full Names:	X-ray repair complementing defective repair in Chinese hamster cells 1
NCBI Official Symbol:	XRCC1
NCBI Official Synonym Symbols:	RCC
NCBI Protein Information:	DNA repair protein XRCC1; X-ray repair cross-complementing protein 1
UniProt Protein Name:	DNA repair protein XRCC1
UniProt Synonym Protein Names:	X-ray repair cross-complementing protein 1
Protein Family:	DNA repair protein
UniProt Gene Name:	XRCC1
UniProt Entry Name:	XRCC1_HUMAN

	Western blot analysis of extracts of various cell lines, using XRCC1 antibody (CAB0442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using XRCC1 antibody (CAB0442) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.