Name: Anti-XPO1 Antibody (CAB0299)
Brand: Immunology Antibodies 1
SKU: CAB0299
Availability: OutOfStock

Antibody Name:	Anti-XPO1 Antibody
Antibody SKU:	CAB0299
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1000 to the C-terminus of human XPO1 (NP_003391.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, B-cell, Mouse liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1000 to the C-terminus of human XPO1 (NP_003391.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GLFS LNQD IPAF KEHL RDFL VQIK EFAG EDTS DLFL EERE IALR QADE EKHK RQMS VPGI FNPH EIPE EMCD
Gene ID:	7514
Uniprot:	O14980
Cellular Location:	Cajal body, Cytoplasm, Nucleus, nucleolus, nucleoplasm
Calculated MW:	123kDa
Observed MW:	123kDa

Synonyms:	CRM1, emb, exp1, XPO1
Background:	This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. The protein specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1.

UniProt Protein Function:	Exportin-1: Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. Belongs to the exportin family.
UniProt Protein Details:	Protein type:Karyopherin; Nuclear import; Nucleolus; Nuclear export Chromosomal Location of Human Ortholog: 2p15 Cellular Component: kinetochore; nucleoplasm; Cajal body; nuclear membrane; intracellular membrane-bound organelle; membrane; cytoplasm; nucleolus; nuclear envelope; cytosol; ribonucleoprotein complex; nucleus; annulate lamellae Molecular Function:protein domain specific binding; protein binding; RNA binding; transporter activity; Ran GTPase binding; protein transporter activity; nucleocytoplasmic transporter activity Biological Process: response to drug; ribosomal small subunit export from nucleus; mRNA transport; viral reproduction; transforming growth factor beta receptor signaling pathway; regulation of protein catabolic process; gene expression; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; protein export from nucleus; negative regulation of transforming growth factor beta receptor signaling pathway; ribosomal large subunit export from nucleus; regulation of protein export from nucleus
NCBI Summary:	This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. The protein specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq, Jan 2015]
UniProt Code:	O14980
NCBI GenInfo Identifier:	68052989
NCBI Gene ID:	7514
NCBI Accession:	O14980.1
UniProt Related Accession:	O14980
Molecular Weight:	123kDa
NCBI Full Name:	Exportin-1
NCBI Synonym Full Names:	exportin 1
NCBI Official Symbol:	XPO1
NCBI Official Synonym Symbols:	emb; CRM1; exp1; CRM-1
NCBI Protein Information:	exportin-1
UniProt Protein Name:	Exportin-1
UniProt Synonym Protein Names:	Chromosome region maintenance 1 protein homolog
Protein Family:	Exportin
UniProt Gene Name:	XPO1
UniProt Entry Name:	XPO1_HUMAN

Western blot analysis of extracts of various cell lines, using XPO1 antibody (CAB0299) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s.

Antibodies

Anti-CRM1 Antibody (CAB19625)

Acetyl-XPO1 (K568) Antibody (PACO06083)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)