Antibody Name:	Anti-XPA Antibody [KO Validated]
Antibody SKU:	CAB1626
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Jurkat, K-562, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA DGAL PEAA ALEQ PAEL PASV RASI ERKR QRAL MLRQ ARLA ARPY SATA AAAT GGMA NVKA APKI IDTG GGFI LEEE EEEE QKIG KVVH QPGP VMEF DYVI CEEC GKEF MDSY LMNH FDLP TCDN CRDA DDKH KLIT KTEA KQEY LLKD CDLE KREP PLKF IVKK NPHH SQWG DMKL YLKL QIVK RSLE VWGS QEAL EEAK EVRQ ENRE KMKQ KKFD KKVK ELRR AVRS SVWK RETI VHQH EYGP EENL EDDM YRKT CTMC GHEL TYEK M
Gene ID:	7507
Uniprot:	P23025
Cellular Location:	Nucleus
Calculated MW:	31kDa
Observed MW:	37kDa

Synonyms:

XPA, XP1, XPAC

Background:

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	XPA: Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. Interacts with GPN1 and RPA1. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2. Expressed in various cell lines and in skin fibroblasts. Belongs to the XPA family.
UniProt Protein Details:	Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 9q22.3 Cellular Component: cytoplasm; DNA replication factor A complex; Golgi apparatus; intercellular bridge; nucleoplasm; nucleus Molecular Function:damaged DNA binding; protein binding; protein domain specific binding; protein homodimerization activity Biological Process: base-excision repair; DNA repair; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; transcription-coupled nucleotide-excision repair Disease: Xeroderma Pigmentosum, Complementation Group A
NCBI Summary:	This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:	P23025
NCBI GenInfo Identifier:	139816
NCBI Gene ID:	7507
NCBI Accession:	P23025.1
UniProt Secondary Accession:	P23025,Q5T1U9, Q6LCW7, Q6LD02,
UniProt Related Accession:	P23025
Molecular Weight:	31,368 Da
NCBI Full Name:	DNA repair protein complementing XP-A cells
NCBI Synonym Full Names:	XPA, DNA damage recognition and repair factor
NCBI Official Symbol:	XPA
NCBI Official Synonym Symbols:	XP1; XPAC
NCBI Protein Information:	DNA repair protein complementing XP-A cells
UniProt Protein Name:	DNA repair protein complementing XP-A cells
UniProt Synonym Protein Names:	Xeroderma pigmentosum group A-complementing protein
Protein Family:	DNA repair protein
UniProt Gene Name:	XPA
UniProt Entry Name:	XPA_HUMAN

	Western blot analysis of extracts of various cell lines, using XPA antibody (CAB1626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and XPA knockout (KO) HeLa cells, using XPA antibody (CAB1626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using XPA antibody (CAB1626). (Green)GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
	Immunofluorescence analysis of A549 cells using XPA antibody (CAB1626).