Cell Biology Antibodies 3

Anti-XDH Antibody (CAB13052)

(No reviews yet) Write a Review
SKU:
CAB13052
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-XDH Antibody
Antibody SKU:CAB13052
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human XDH (NP_000370.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human XDH (NP_000370.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTAD KLVF FVNG RKVV EKNA DPET TLLA YLRR KLGL SGTK LGCG EGGC GACT VMLS KYDR LQNK IVHF SANA CLAP ICSL HHVA VTTV EGIG STKT RLHP VQER IAKS HGSQ CGFC TPGI VMSM YTLL RNQP EPTM EEIE NAFQ GNLC RCTG YRPI LQGF RTFA RDGG CCGG DGNN PNCC MNQK KDHS VSLS PSLF KPEE FTPL DPTQ EPIF PPEL LRLK DTPR KQLR FEGE RVTW IQAS TLKE LLDL KAQH PDAK LVVG NTEI GIEM KFKN MLFP MIVC
Gene ID:7498
Uniprot:P47989
Cellular Location:Cytoplasm, Peroxisome, Secreted
Calculated MW:146kDa
Observed MW:146kDa
Synonyms:XDH, XAN1, XO, XOR
Background:Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
UniProt Protein Function:XDH: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Defects in XDH are the cause of xanthinuria type 1 (XU1). Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. Belongs to the xanthine dehydrogenase family.
UniProt Protein Details:

Protein type:EC 1.17.1.4; Secondary Metabolites Metabolism - caffeine; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - purine; EC 1.17.3.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p23.1

Cellular Component: cytosol; extracellular space

Molecular Function:2 iron, 2 sulfur cluster binding; FAD binding; molybdopterin cofactor binding; oxidoreductase activity, acting on the aldehyde or oxo group of donors; protein homodimerization activity; xanthine dehydrogenase activity; xanthine oxidase activity

Biological Process: caspase activation; negative regulation of endothelial cell differentiation; negative regulation of endothelial cell proliferation; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase B signaling cascade; purine nucleotide catabolic process; xanthine catabolic process

Disease: Xanthinuria, Type I

NCBI Summary:Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]
UniProt Code:P47989
NCBI GenInfo Identifier:2506326
NCBI Gene ID:7498
NCBI Accession:P47989.4
UniProt Secondary Accession:P47989,Q16681, Q16712, Q4PJ16,
UniProt Related Accession:P47989
Molecular Weight:146,424 Da
NCBI Full Name:Xanthine dehydrogenase/oxidase
NCBI Synonym Full Names:xanthine dehydrogenase
NCBI Official Symbol:XDH  
NCBI Official Synonym Symbols:XO; XOR  
NCBI Protein Information:xanthine dehydrogenase/oxidase
UniProt Protein Name:Xanthine dehydrogenase/oxidase
UniProt Synonym Protein Names:Xanthine oxidoreductase; XOR
Protein Family:Xanthine dehydrogenase
UniProt Gene Name:XDH  
UniProt Entry Name:XDH_HUMAN
Anti-XDH Antibody (CAB13052)Western blot analysis of extracts of mouse liver, using XDH antibody (CAB13052) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
View AllClose

0 Reviews

View AllClose

Related Products