Immunology Antibodies 2

Anti-WWP2 Antibody (CAB6789)

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SKU:
CAB6789
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-WWP2 Antibody
Antibody SKU:CAB6789
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WWP2 (NP_001257383.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:HL-60, Rat pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WWP2 (NP_001257383.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MASA SSSR AGVA LPFE KSQL TLKV VSAK PKVH NRQP RINS YVEV AVDG LPSE TKKT GKRI GSSE LLWN EIII LNVT AQSH LDLK VWSC HTLR NELL GTAS VNLS NVLK NNGG KMEN MQLT LNLQ TENK GSVV SGGE LTIF LDGP TVDL GNVP NGSA LTDG SQLP SRDS SGTA VAPE NRHQ PPST NCFG GRSR THRH SGAS ARTT PATG EQSP GARS RHRQ PVKN SGHS GLAN GTVN DEPT TATD PEEP SVVG VTSP PAAP LSVT PNPN TT
Gene ID:11060
Uniprot:O00308
Cellular Location:Nucleus
Calculated MW:35kDa/51kDa/86kDa/98kDa
Observed MW:115-120kDa
Synonyms:WWP2, AIP2, WWp2-like
Background:This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10.
UniProt Protein Function:WWP2: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation- induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: membrane; cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function:protein binding; ubiquitin-protein ligase activity; transcription factor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; entry of virus into host cell; regulation of membrane potential; protein autoubiquitination; negative regulation of transcription factor activity; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; protein modification process; negative regulation of protein transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transporter activity

NCBI Summary:This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012]
UniProt Code:O00308
NCBI GenInfo Identifier:32171765
NCBI Gene ID:11060
NCBI Accession:O00308.2
UniProt Secondary Accession:O00308,Q6ZTQ5, Q96CZ2, Q9BWN6, A6NEP1, B2R706, B4DTL5 F5H213, H3BRF3, I3RSG8,
UniProt Related Accession:O00308
Molecular Weight:Observed MW 105kDaCalculated MW 35kDa/51kDa/86kDa/98kDa
NCBI Full Name:NEDD4-like E3 ubiquitin-protein ligase WWP2
NCBI Synonym Full Names:WW domain containing E3 ubiquitin protein ligase 2
NCBI Official Symbol:WWP2  
NCBI Official Synonym Symbols:AIP2; WWp2-like  
NCBI Protein Information:NEDD4-like E3 ubiquitin-protein ligase WWP2; atrophin-1 interacting protein 2
UniProt Protein Name:NEDD4-like E3 ubiquitin-protein ligase WWP2
UniProt Synonym Protein Names:Atrophin-1-interacting protein 2; AIP2; WW domain-containing protein 2
Protein Family:NEDD4-like E3 ubiquitin-protein ligase
UniProt Gene Name:WWP2  
UniProt Entry Name:WWP2_HUMAN
Anti-WWP2 Antibody (CAB6789)Western blot analysis of extracts of various cell lines, using WWP2 antibody (CAB6789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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system_update_altDatasheetHuman WWP2 Recombinant Protein WWP2 contains 1 C2 domain, 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain and 4 WW domains. It is an E3 ubiquitin-protein ligase wh