Antibody Name:	Anti-WWOX Antibody
Antibody SKU:	CAB12652
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human WWOX (NP_057457.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SKOV3, LO2, HT-29, A-549, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human WWOX (NP_057457.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAL RYAG LDDT DSED ELPP GWEE RTTK DGWV YYAN HTEE KTQW EHPK TGKR KRVA GDLP YGWE QETD ENGQ VFFV DHIN KRTT YLDP RLAF TVDD NPTK PTTR QRYD GSTT AMEI LQGR
Gene ID:	51741
Uniprot:	Q9NZC7
Cellular Location:	Cytoplasm, Golgi apparatus, Mitochondrion, Nucleus
Calculated MW:	4kDa/21kDa/23kDa/26kDa/35kDa/41kDa/46kDa
Observed MW:	46kDa

Synonyms:

WWOX, D16S432E, EIEE28, FOR, FRA16D, HHCMA56, PRO0128, SCAR12, SDR41C1, WOX1

Background:

This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	WWOX: an enzyme which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). Expressed at high levels in hormonally regulated tissues such as testis, ovary, and prostate. Its SRD domain suggest a role for this gene in steroid metabolism. Shown to be an essential mediator of tumor necrosis factor-alpha-induced apoptosis in the mouse. May play a similar role in the human. May function as a suppressor of tumor growth. Alternative splicing of this gene generates 7 isoforms.
UniProt Protein Details:	Protein type:Tumor suppressor; Oxidoreductase; Apoptosis; EC 1.1.1.- Chromosomal Location of Human Ortholog: 16q23 Cellular Component: cytoplasm; cytosol; Golgi apparatus; microvillus; mitochondrion; nucleus; plasma membrane Molecular Function:coenzyme binding; cofactor binding; enzyme binding; oxidoreductase activity; protein binding; protein dimerization activity Biological Process: negative regulation of Wnt receptor signaling pathway; osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; skeletal morphogenesis; steroid metabolic process; Wnt receptor signaling pathway Disease: Epileptic Encephalopathy, Early Infantile, 28
NCBI Summary:	This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:	Q9NZC7
NCBI GenInfo Identifier:	74725363
NCBI Gene ID:	51741
NCBI Accession:	Q9NZC7.1
UniProt Secondary Accession:	Q9NZC7,Q5MYT5, Q96KM3, Q96RF2, Q9BTT8, Q9NPC9, Q9NRF4 Q9NRF5, Q9NRF6, Q9NRK1, Q9NZC5, A8K323,
UniProt Related Accession:	Q9NZC7
Molecular Weight:	23,868 Da
NCBI Full Name:	WW domain-containing oxidoreductase
NCBI Synonym Full Names:	WW domain containing oxidoreductase
NCBI Official Symbol:	WWOX
NCBI Official Synonym Symbols:	FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
NCBI Protein Information:	WW domain-containing oxidoreductase
UniProt Protein Name:	WW domain-containing oxidoreductase
UniProt Synonym Protein Names:	Fragile site FRA16D oxidoreductase; Short chain dehydrogenase/reductase family 41C member 1
Protein Family:	WW domain-containing oxidoreductase
UniProt Gene Name:	WWOX
UniProt Entry Name:	WWOX_HUMAN

	Western blot analysis of extracts of various cell lines, using WWOX antibody (CAB12652) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of NIH-3T3 cells using WWOX Polyclonal Antibody (CAB12652) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.