Cell Cycle Antibodies 1
Anti-WTAP Antibody (CAB14695)
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- SKU:
- CAB14695
- Product Type:
- Antibody
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-WTAP Antibody |
| Antibody SKU: | CAB14695 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-396 of human WTAP (NP_004897.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-396 of human WTAP (NP_004897.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTNE EPLP KKVR LSET DFKV MARD ELIL RWKQ YEAY VQAL EGKY TDLN SNDV TGLR ESEE KLKQ QQQE SARR ENIL VMRL ATKE QEMQ ECTT QIQY LKQV QQPS VAQL RSTM VDPA INLF FLKM KGEL EQTK DKLE QAQN ELSA WKFT PDSQ TGKK LMAK CRML IQEN QELG RQLS QGRI AQLE AELA LQKK YSEE LKSS QDEL NDFI IQLD EEVE GMQS TILV LQQQ LKET RQQL AQYQ QQQS QASA PSTS RTTA SEPV EQSE ATSK DCSR LTNG PSNG SSSR QRTS GSGF HREG NTTE DDFP SSPG NGNK SSNS SEER TGRG GSGY VNQL SAGY ESVD SPTG SENS LTHQ SNDT DSSH DPQE EKAV SGKG NRTV GSRH VQNG LDSS VNVQ GSVL |
| Gene ID: | 9589 |
| Uniprot: | Q15007 |
| Cellular Location: | Nucleus, Nucleus speckle, nucleoplasm |
| Calculated MW: | 17kDa/44kDa |
| Observed MW: | 44kDa |
| Synonyms: | WTAP, Mum2 |
| Background: | The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. |
| UniProt Protein Function: | WTAP: Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA- binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation. Belongs to the fl(2)d family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nucleolus; Spliceosome Chromosomal Location of Human Ortholog: 6q25-q27 Cellular Component: nuclear membrane; nuclear speck; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: gene expression; regulation of alternative nuclear mRNA splicing, via spliceosome |
| NCBI Summary: | The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012] |
| UniProt Code: | Q15007 |
| NCBI GenInfo Identifier: | 47117889 |
| NCBI Gene ID: | 9589 |
| NCBI Accession: | Q15007.2 |
| UniProt Secondary Accession: | Q15007,Q5TCL8, Q5TCL9, Q96T28, Q9BYJ7, Q9H4E2, |
| UniProt Related Accession: | Q15007 |
| Molecular Weight: | 17,801 Da |
| NCBI Full Name: | Pre-mRNA-splicing regulator WTAP |
| NCBI Synonym Full Names: | Wilms tumor 1 associated protein |
| NCBI Official Symbol: | WTAP |
| NCBI Official Synonym Symbols: | Mum2 |
| NCBI Protein Information: | pre-mRNA-splicing regulator WTAP |
| UniProt Protein Name: | Pre-mRNA-splicing regulator WTAP |
| UniProt Synonym Protein Names: | Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein |
| Protein Family: | Pre-mRNA-splicing regulator |
| UniProt Gene Name: | WTAP |
| UniProt Entry Name: | FL2D_HUMAN |
 | Western blot analysis of extracts of rat spleen, using WTAP antibody (CAB14695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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