Name: Anti-WRN Antibody (CAB6855)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB6855
Availability: OutOfStock

Antibody Name:	Anti-WRN Antibody
Antibody SKU:	CAB6855
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1223-1432 of human WRN (NP_000544.2).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:1000
Reactivity:	Human
Positive Samples:	DU145, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1223-1432 of human WRN (NP_000544.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CQTN SVQT DLFS STKP QEEQ KTSL VAKN KICT LSQS MAIT YSLF QEKK MPLK SIAE SRIL PLMT IGMH LSQA VKAG CPLD LERA GLTP EVQK IIAD VIRN PPVN SDMS KISL IRML VPEN IDTY LIHM AIEI LKHG PDSG LQPS CDVN KRRC FPGS EEIC SSSK RSKE EVGI NTET SSAE RKRR LPVW FAKG SDTS KKLM DKTK RGGL FS
Gene ID:	7486
Uniprot:	Q14191
Cellular Location:	Nucleus, nucleolus, nucleoplasm
Calculated MW:	162kDa
Observed MW:	200kDa

Synonyms:

WRN, RECQ3, RECQL2, RECQL3

Background:

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

UniProt Protein Function:	WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.
UniProt Protein Details:	Protein type:DNA repair, damage; DNA-binding; Helicase; EC 3.6.4.12; Cell cycle regulation; Nucleolus Chromosomal Location of Human Ortholog: 8p12 Cellular Component: centrosome; cytoplasm; MutLalpha complex; nuclear chromosome, telomeric region; nucleolus; nucleoplasm Molecular Function:3'-5' DNA helicase activity; 3'-5' exonuclease activity; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATPase activity; bubble DNA binding; DNA binding; DNA helicase activity; exonuclease activity; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; magnesium ion binding; manganese ion binding; protein binding; protein complex binding; protein homodimerization activity; Y-form DNA binding Biological Process: base-excision repair; cell aging; cellular response to starvation; DNA metabolic process; DNA replication; DNA synthesis during DNA repair; double-strand break repair; multicellular organismal aging; nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; protein sumoylation; regulation of apoptosis; replication fork processing; response to DNA damage stimulus; response to oxidative stress; response to UV-C; strand displacement; telomere maintenance; telomere maintenance via recombination Disease: Werner Syndrome
NCBI Summary:	This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14191
NCBI GenInfo Identifier:	322510082
NCBI Gene ID:	7486
NCBI Accession:	Q14191.2
UniProt Secondary Accession:	Q14191,A1KYY9,
UniProt Related Accession:	Q14191
Molecular Weight:	162,461 Da
NCBI Full Name:	Werner syndrome ATP-dependent helicase
NCBI Synonym Full Names:	Werner syndrome RecQ like helicase
NCBI Official Symbol:	WRN
NCBI Official Synonym Symbols:	RECQ3; RECQL2; RECQL3
NCBI Protein Information:	Werner syndrome ATP-dependent helicase
UniProt Protein Name:	Werner syndrome ATP-dependent helicase
UniProt Synonym Protein Names:	DNA helicase, RecQ-like type 3; RecQ3; Exonuclease WRN (EC:3.1.-.-); RecQ protein-like 2
Protein Family:	Werner syndrome ATP-dependent helicase
UniProt Gene Name:	WRN
UniProt Entry Name:	WRN_HUMAN

Western blot analysis of extracts of various cell lines, using WRN antibody (CAB6855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

Phospho-WRN (S1141) Antibody (PACO05311)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)