Cell Biology Antibodies 10
Anti-WRB Antibody (CAB7000)
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- SKU:
- CAB7000
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-WRB Antibody |
| Antibody SKU: | CAB7000 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human WRB (NP_004618.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse testis, Mouse skin, Rat lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human WRB (NP_004618.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SFSS FMSR VLQK DAEQ ESQM RAEI QDMK QELS TVNM MDEF ARYA RLER KINK MTDK LKTH VKAR TAQL AKI |
| Gene ID: | 7485 |
| Uniprot: | O00258 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 16kDa/19kDa |
| Observed MW: | 25kDa |
| Synonyms: | WRB, CHD5, GET1 |
| Background: | This gene encodes a basic nuclear protein of unknown function. The gene is widely expressed in adult and fetal tissues. Since the region proposed to contain the gene(s) for congenital heart disease (CHD) in Down syndrome (DS) patients has been restricted to 21q22.2-22.3, this gene, which maps to 21q22.3, has a potential role in the pathogenesis of Down syndrome congenital heart disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | WRB: Receptor for ASNA1/TRC40-mediated insertion of tail- anchored (TA) proteins into the ER membrane. Belongs to the WRB/GET1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: endoplasmic reticulum membrane; integral to membrane; nucleus |
| NCBI Summary: | This gene encodes a basic nuclear protein of unknown function. The gene is widely expressed in adult and fetal tissues. Since the region proposed to contain the gene(s) for congenital heart disease (CHD) in Down syndrome (DS) patients has been restricted to 21q22.2-22.3, this gene, which maps to 21q22.3, has a potential role in the pathogenesis of Down syndrome congenital heart disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009] |
| UniProt Code: | O00258 |
| NCBI GenInfo Identifier: | 12643986 |
| NCBI Gene ID: | 7485 |
| NCBI Accession: | O00258.2 |
| UniProt Secondary Accession: | O00258,O60740, A8KAP8, A8MQ44, D3DSH9, |
| UniProt Related Accession: | O00258 |
| Molecular Weight: | 174 |
| NCBI Full Name: | Tail-anchored protein insertion receptor WRB |
| NCBI Synonym Full Names: | tryptophan rich basic protein |
| NCBI Official Symbol: | WRB |
| NCBI Official Synonym Symbols: | CHD5 |
| NCBI Protein Information: | tail-anchored protein insertion receptor WRB; tryptophan-rich protein; tryptophan-rich basic protein; congenital heart disease 5 protein |
| UniProt Protein Name: | Tail-anchored protein insertion receptor WRB |
| UniProt Synonym Protein Names: | Congenital heart disease 5 protein; Tryptophan-rich basic protein; WRB |
| Protein Family: | Tail-anchored protein insertion receptor |
| UniProt Gene Name: | WRB |
| UniProt Entry Name: | WRB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WRB antibody (CAB7000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using WRB antibody (CAB7000) at dilution of 1:200 (40x lens). |
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