Antibody Name:	Anti-WNT9A Antibody
Antibody SKU:	CAB7939
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 206-365 of human WNT9A (NP_003386.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 206-365 of human WNT9A (NP_003386.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VIKA GVET TCKC HGVS GSCT VRTC WRQL APFH EVGK HLKH KYET ALKV GSTT NEAA GEAG AISP PRGR ASGA GGSD PLPR TPEL VHLD DSPS FCLA GRFS PGTA GRRC HREK NCES ICCG RGHN TQSR VVTR PCQC QVRW CCYV ECRQ CTQR EEVY TCKG
Gene ID:	7483
Uniprot:	O14904
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	40kDa
Observed MW:	30kDa

Synonyms:

WNT9A, WNT14

Background:

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region.

UniProt Protein Function:	WNT9A: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1q42 Cellular Component: extracellular region; extracellular space Molecular Function:frizzled binding Biological Process: cell fate commitment; mitotic cell cycle checkpoint; negative regulation of cell proliferation; negative regulation of chondrocyte differentiation; neuron differentiation; Wnt receptor signaling pathway
NCBI Summary:	The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region. [provided by RefSeq, Jul 2008]
UniProt Code:	O14904
NCBI GenInfo Identifier:	20532420
NCBI Gene ID:	7483
NCBI Accession:	O14904.2
UniProt Secondary Accession:	O14904,Q2M2J3, Q5VWU0, Q96S50, A6NLW2,
UniProt Related Accession:	O14904
Molecular Weight:	40,320 Da
NCBI Full Name:	Protein Wnt-9a
NCBI Synonym Full Names:	Wnt family member 9A
NCBI Official Symbol:	WNT9A
NCBI Official Synonym Symbols:	WNT14
NCBI Protein Information:	protein Wnt-9a
UniProt Protein Name:	Protein Wnt-9a
UniProt Synonym Protein Names:	Protein Wnt-14
Protein Family:	Protein
UniProt Gene Name:	WNT9A
UniProt Entry Name:	WNT9A_HUMAN

	Western blot analysis of extracts of mouse lung, using WNT9A antibody (CAB7939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using WNT9A antibody (CAB7939) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using WNT9A antibody (CAB7939) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using WNT9A antibody (CAB7939) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using WNT9A antibody (CAB7939) at dilution of 1:100 (40x lens).