Antibody Name:	Anti-WNT7A Antibody
Antibody SKU:	CAB5425
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 32-170 of human WNT7A (NP_004616.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 32-170 of human WNT7A (NP_004616.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LGAS IICN KIPG LAPR QRAI CQSR PDAI IVIG EGSQ MGLD ECQF QFRN GRWN CSAL GERT VFGK ELKV GSRE AAFT YAII AAGV AHAI TAAC TQGN LSDC GCDK EKQG QYHR DEGW KWGG CSAD IRYG IGFA KVFV DAR
Gene ID:	7476
Uniprot:	O00755
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	39kDa
Observed MW:	39kDa

Synonyms:

WNT7A

Background:

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes.

UniProt Protein Function:	WNT7A: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. Defects in WNT7A are the cause of limb pelvis hypoplasia aplasia syndrome (LPHAS). A syndrome of severe deficiency of the extremities due to hypo- or aplasia of one or more long bones of one or more limbs. Pelvic manifestations include hip dislocation, hypoplastic iliac bone and aplastic pubic bones. Thoracic deformity, unusual facies and genitourinary anomalies can be present. Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS); also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. Belongs to the Wnt family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3p25 Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region Molecular Function:frizzled binding; cytokine activity; receptor agonist activity; receptor binding Biological Process: embryonic forelimb morphogenesis; somatic stem cell maintenance; positive regulation of epithelial cell proliferation involved in wound healing; cell proliferation in forebrain; neurotransmitter secretion; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; Wnt receptor signaling pathway through beta-catenin; palate development; embryonic hindlimb morphogenesis; response to estradiol stimulus; negative regulation of neurogenesis; neuron differentiation; central nervous system vasculogenesis; regulation of axon diameter; synapse organization and biogenesis; somatic stem cell division; chondrocyte differentiation; satellite cell activation; angiogenesis; fallopian tube development; cartilage condensation; cell fate commitment; embryonic axis specification; satellite cell compartment self-renewal involved in skeletal muscle regeneration; cerebellar granule cell differentiation; asymmetric protein localization; positive regulation of synaptogenesis; stem cell development; dorsal/ventral pattern formation; establishment of cell polarity; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; sex differentiation; negative regulation of apoptosis Disease: Ulna And Fibula, Absence Of, With Severe Limb Deficiency; Fibular Aplasia Or Hypoplasia, Femoral Bowing And Poly-, Syn-, And Oligodactyly
NCBI Summary:	This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq, Jul 2008]
UniProt Code:	O00755
NCBI GenInfo Identifier:	145559540
NCBI Gene ID:	7476
NCBI Accession:	O00755.2
UniProt Secondary Accession:	O00755,Q96H90, Q9Y560,
UniProt Related Accession:	O00755
Molecular Weight:	349
NCBI Full Name:	Protein Wnt-7a
NCBI Synonym Full Names:	wingless-type MMTV integration site family, member 7A
NCBI Official Symbol:	WNT7A
NCBI Protein Information:	protein Wnt-7a; proto-oncogene Wnt7a protein
UniProt Protein Name:	Protein Wnt-7a
UniProt Gene Name:	WNT7A
UniProt Entry Name:	WNT7A_HUMAN

	Western blot analysis of extracts of mouse kidney, using WNT7A antibody (CAB5425). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of A-549 cells using WNT7A antibody (CAB5425).