Cell Biology Antibodies 16
Anti-WNK3 Antibody (CAB5574)
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- SKU:
- CAB5574
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-WNK3 Antibody |
| Antibody SKU: | CAB5574 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of rat WNK3 |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Rat hypothalamus, Rat CNS, Rat brain |
| Immunogen: | Recombinant protein of rat WNK3 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 317420 |
| Uniprot: | |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 198kDa |
| Observed MW: | 40kDa |
| Synonyms: | WNK3, PRKWNK3 |
| Background: | This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. |
 | Western blot analysis of extracts of various cell lines, using WNK3 antibody (CAB5574). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using WNK3 antibody (CAB5574) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human appendix using WNK3 antibody (CAB5574) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using WNK3 antibody (CAB5574) at dilution of 1:100 (40x lens). |
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