Cell Biology Antibodies 6
Anti-WNK3 Antibody (CAB15507)
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- SKU:
- CAB15507
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-WNK3 Antibody |
| Antibody SKU: | CAB15507 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-600 of human WNK3 (NP_065973.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-600 of human WNK3 (NP_065973.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AGCL EERR DSQC KSMG NVFP QPQN TTLP LAPA QQTG AECE ETEV DQHV RQQL LQRK PQQH CSSV TGDN LSEA GAAS VIHS DTSS QPSV AYSS NQTM GSQM V |
| Gene ID: | 65267 |
| Uniprot: | Q9BYP7 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 191kDa/192kDa/197kDa/198kDa |
| Observed MW: | 198kDa |
| Synonyms: | WNK3, PRKWNK3 |
| Background: | This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | WNK3: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; Wnk family Chromosomal Location of Human Ortholog: Xp11.22 Cellular Component: adherens junction; cytoplasm; cytosol; tight junction Molecular Function:ATP binding; chloride channel inhibitor activity; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: negative regulation of apoptosis; positive regulation of calcium ion transport; positive regulation of ion transmembrane transporter activity; protein amino acid autophosphorylation; protein amino acid phosphorylation |
| NCBI Summary: | This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
| UniProt Code: | Q9BYP7 |
| NCBI GenInfo Identifier: | 353526307 |
| NCBI Gene ID: | 65267 |
| NCBI Accession: | Q9BYP7.3 |
| UniProt Secondary Accession: | Q9BYP7,Q5JRC1, Q6JP76, Q8TCX6, Q9HCK6, B1AKG2, |
| UniProt Related Accession: | Q9BYP7 |
| Molecular Weight: | 192,875 Da |
| NCBI Full Name: | Serine/threonine-protein kinase WNK3 |
| NCBI Synonym Full Names: | WNK lysine deficient protein kinase 3 |
| NCBI Official Symbol: | WNK3 |
| NCBI Official Synonym Symbols: | PRKWNK3 |
| NCBI Protein Information: | serine/threonine-protein kinase WNK3 |
| UniProt Protein Name: | Serine/threonine-protein kinase WNK3 |
| UniProt Synonym Protein Names: | Protein kinase lysine-deficient 3; Protein kinase with no lysine 3 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | WNK3 |
| UniProt Entry Name: | WNK3_HUMAN |
 | Western blot analysis of extracts of mouse brain, using WNK3 antibody (CAB15507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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