Cell Biology Antibodies 8
Anti-WISP1 Antibody (CAB3346)
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- SKU:
- CAB3346
Description
| Antibody Name: | Anti-WISP1 Antibody |
| Antibody SKU: | CAB3346 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 158-367 of human WISP1 (NP_003873.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, A-549, SGC-7901, HeLa, Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 158-367 of human WISP1 (NP_003873.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LRVR PPRL WCPH PRRV SIPG HCCE QWVC EDDA KRPR KTAP RDTG AFDA VGEV EAWH RNCI AYTS PWSP CSTS CGLG VSTR ISNV NAQC WPEQ ESRL CNLR PCDV DIHT LIKA GKKC LAVY QPEA SMNF TLAG CIST RSYQ PKYC GVCM DNRC CIPY KSKT IDVS FQCP DGLG FSRQ VLWI NACF CNLS CRNP NDIF ADLE SYPD FSEI AN |
| Gene ID: | 8840 |
| Uniprot: | O95388 |
| Cellular Location: | Secreted |
| Calculated MW: | 13kDa/16kDa/21kDa/30kDa/40kDa |
| Observed MW: | 45kDa |
| Synonyms: | WISP1, CCN4, WISP1c, WISP1i, WISP1tc |
| Background: | This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. |
| UniProt Protein Function: | Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. |
| NCBI Summary: | This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011] |
| UniProt Code: | O95388 |
| NCBI GenInfo Identifier: | 34098393 |
| NCBI Gene ID: | 8840 |
| NCBI Accession: | O95388.1 |
| UniProt Secondary Accession: | O95388,Q5JBS6, Q5JBS7, Q5JBS8, Q9HCS3, A8KAG6, E7EMM5 |
| UniProt Related Accession: | O95388 |
| Molecular Weight: | 21,550 Da |
| NCBI Full Name: | WNT1-inducible-signaling pathway protein 1 |
| NCBI Synonym Full Names: | WNT1 inducible signaling pathway protein 1 |
| NCBI Official Symbol: | WISP1 |
| NCBI Official Synonym Symbols: | CCN4; WISP1c; WISP1i; WISP1tc |
| NCBI Protein Information: | WNT1-inducible-signaling pathway protein 1 |
| UniProt Protein Name: | WNT1-inducible-signaling pathway protein 1 |
| UniProt Synonym Protein Names: | CCN family member 4; Wnt-1-induced secreted protein |
| Protein Family: | WNT1-inducible-signaling pathway protein |
| UniProt Gene Name: | WISP1 |
| UniProt Entry Name: | WISP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WISP1 antibody (CAB3346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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Additional Information
Product Type: |
Antibody |
Reactivity: |
Human |
Reactivity: |
Mouse |
Host Species: |
Rabbit |
Isotype: |
IgG |
Research Area: |
Cell Biology |
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