Autophagy Antibodies

Anti-WIPI2 Antibody (CAB7537)

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SKU:
CAB7537
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy

Description

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Antibody Name:Anti-WIPI2 Antibody
Antibody SKU:CAB7537
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human WIPI2 (NP_057087.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Jurkat, BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human WIPI2 (NP_057087.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LAAL AFDA SGTK LATA SEKG TVIR VFSI PEGQ KLFE FRRG VKRC VSIC SLAF SMDG MFLS ASSN TETV HIFK LETV KEKP PEEP TTWT GYFG KVLM ASTS YLPS QVTE MFNQ GRAF ATVR LPFC GHKN ICSL ATIQ KIPR LLVG AADG YLYM YNLD PQEG GECA LMKQ HRLD GSLE TTNE ILDS ASHD CPLV TQTY GAAA GKGT YVPS SPTR LAYT DDLG AVGG ACLE DEAS ALRL DEDS EHPP MILR TD
Gene ID:26100
Uniprot:Q9Y4P8
Cellular Location:Cytoplasmic side, Peripheral membrane protein, Preautophagosomal structure membrane
Calculated MW:42kDa/46kDa/47kDa/48kDa/49kDa
Observed MW:48kDa
Synonyms:WIPI2, ATG18B, Atg21, CGI-50, WIPI-2
Background:
UniProt Protein Function:Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.
NCBI Summary:WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9Y4P8
NCBI GenInfo Identifier:74762063
NCBI Gene ID:26100
NCBI Accession:Q9Y4P8.1
UniProt Secondary Accession:Q9Y4P8,Q5MNZ8, Q6FI96, Q75L50, Q96IE4, Q9Y364, B3KNC2
UniProt Related Accession:Q9Y4P8
Molecular Weight:49kDa
NCBI Full Name:WD repeat domain phosphoinositide-interacting protein 2
NCBI Synonym Full Names:WD repeat domain, phosphoinositide interacting 2
NCBI Official Symbol:WIPI2  
NCBI Official Synonym Symbols:Atg21; ATG18B; CGI-50; WIPI-2  
NCBI Protein Information:WD repeat domain phosphoinositide-interacting protein 2
UniProt Protein Name:WD repeat domain phosphoinositide-interacting protein 2
UniProt Synonym Protein Names:WIPI49-like protein 2
Protein Family:WD repeat domain phosphoinositide-interacting protein
UniProt Gene Name:WIPI2  
Anti-WIPI2 Antibody (CAB7537)Western blot analysis of extracts of various cell lines, using WIPI2 antibody (CAB7537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-WIPI2 Antibody (CAB7537)Immunohistochemistry of paraffin-embedded human kidney using WIPI2 antibody (CAB7537) at dilution of 1:100 (40x lens).
Anti-WIPI2 Antibody (CAB7537)Immunohistochemistry of paraffin-embedded mouse kidney using WIPI2 antibody (CAB7537) at dilution of 1:100 (40x lens).
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