Metabolism Antibodies 2
Anti-WIF1 Antibody (CAB5386)
- SKU:
- CAB5386
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-WIF1 Antibody |
| Antibody SKU: | CAB5386 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-220 of human WIF1 (NP_009122.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse trachea, Mouse heart, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-220 of human WIF1 (NP_009122.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GPPQ EESL YLWI DAHQ ARVL IGFE EDIL IVSE GKMA PFTH DFRK AQQR MPAI PVNI HSMN FTWQ AAGQ AEYF YEFL SLRS LDKG IMAD PTVN VPLL GTVP HKAS VVQV GFPC LGKQ DGVA AFEV DVIV MNSE GNTI LQTP QNAI FFKT CQQA ECPG GCRN GGFC NERR ICEC PDGF HGPH CEKA LCTP RCMN |
| Gene ID: | 11197 |
| Uniprot: | Q9Y5W5 |
| Cellular Location: | Secreted |
| Calculated MW: | 41kDa |
| Observed MW: | 39kDa |
| Synonyms: | WIF1, WIF-1 |
| Background: | The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. |
| UniProt Protein Function: | WIF1: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. |
| UniProt Protein Details: | Protein type:Inhibitor; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 12q14.3 Molecular Function:protein binding |
| NCBI Summary: | The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010] |
| UniProt Code: | Q9Y5W5 |
| NCBI GenInfo Identifier: | 61252765 |
| NCBI Gene ID: | 11197 |
| NCBI Accession: | Q9Y5W5.3 |
| UniProt Secondary Accession: | Q9Y5W5,Q6UXI1, Q8WVG4, |
| UniProt Related Accession: | Q9Y5W5 |
| Molecular Weight: | 41,528 Da |
| NCBI Full Name: | Wnt inhibitory factor 1 |
| NCBI Synonym Full Names: | WNT inhibitory factor 1 |
| NCBI Official Symbol: | WIF1 |
| NCBI Official Synonym Symbols: | WIF-1 |
| NCBI Protein Information: | wnt inhibitory factor 1 |
| UniProt Protein Name: | Wnt inhibitory factor 1 |
| Protein Family: | Wnt inhibitory factor |
| UniProt Gene Name: | WIF1 |
| UniProt Entry Name: | WIF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WIF1 antibody (CAB5386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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