Name: Anti-WHSC1L1 Antibody (CAB5577)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB5577
Availability: OutOfStock

Antibody Name:	Anti-WHSC1L1 Antibody
Antibody SKU:	CAB5577
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WHSC1L1 (NP_060248.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	293T, HepG2, HeLa, MCF7, Mouse liver, Mouse brain, Mouse spleen, Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WHSC1L1 (NP_060248.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDFS FSFM QGIM GNTI QQPP QLID SANI RQED AFDN NSDI AEDG GQTP YEAT LQQG FQYP ATTE DLPP LTNG YPSS ISVY ETQT KYQS YNQY PNGS ANGF GAVR NFSP TDYY HSEI PNTR PHEI LEKP SPPQ PPPP PSVP QTVI PKKT GSPE IKLK ITKT IQNG RELF ESSL CGDL LNEV QASE HTKS KHES RKEK RKKS NKHD SSRS EERK SHKI PKLE PEEQ NRPN ERVD TVSE KPRE EPVL KEEA PVQP ILSS VPTT EVST GVKF QV
Gene ID:	54904
Uniprot:	Q9BZ95
Cellular Location:	Chromosome, Nucleus
Calculated MW:	72kDa/154kDa/156kDa/160kDa/161kDa
Observed MW:	80-90kDa

Synonyms:	NSD3, KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328
Background:	This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described.

UniProt Protein Function:	WHSC1L1: is related to the Wolf-Hirschhorn syndrome candidate-1 protein containing PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; Oncoprotein; Histone-binding; EC 2.1.1.43; Methyltransferase Chromosomal Location of Human Ortholog: 8p11.2 Cellular Component: nucleoplasm; chromosome; nucleus Molecular Function:protein binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific) Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; histone H3-K4 methylation; negative regulation of transcription, DNA-dependent Disease: Leukemia, Acute Myeloid
NCBI Summary:	This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015]
UniProt Code:	Q9BZ95
NCBI GenInfo Identifier:	74761342
NCBI Gene ID:	54904
NCBI Accession:	Q9BZ95.1
UniProt Secondary Accession:	Q9BZ95,Q6P2L6,
UniProt Related Accession:	Q9BZ95
Molecular Weight:	162kDa
NCBI Full Name:	Histone-lysine N-methyltransferase NSD3
NCBI Synonym Full Names:	nuclear receptor binding SET domain protein 3
NCBI Official Symbol:	NSD3
NCBI Official Synonym Symbols:	KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328
NCBI Protein Information:	histone-lysine N-methyltransferase NSD3
UniProt Protein Name:	Histone-lysine N-methyltransferase NSD3
UniProt Synonym Protein Names:	Nuclear SET domain-containing protein 3; Protein whistle; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; Wolf-Hirschhorn syndrome candidate 1-like protein 1; WHSC1-like protein 1
UniProt Gene Name:	WHSC1L1
UniProt Entry Name:	NSD3_HUMAN

	Western blot analysis of extracts of various cell lines, using WHSC1L1 antibody (CAB5577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using WHSC1L1 antibody (CAB5577). Blue: DAPI for nuclear staining.