Antibody Name:	Anti-WFS1 Antibody
Antibody SKU:	CAB1705
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human WFS1 (NP_001139325.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, HeLa, Mouse heart, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human WFS1 (NP_001139325.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDSN TAPL GPSC PQPP PAPQ PQAR SRLN ATAS LEQE RSER PRAP GPQA GPGP GVRD AAAP AEPQ AQHT RSRE RADG TGPT KGDM EIPF EEVL ERAK AGDP KAQT EVGK HYLQ LAGD TDEE LNSC TAVD WLVL AAKQ GRRE AVKL LRRC LADR RGIT SENE REVR QLSS ETDL ERAV RKAA LVMY WKLN PKKK KQVA VAEL LENV GQVN EHDG GAQP GPVP KSLQ KQRR MLER LVSS ESKN YIAL DDFV EITK KYAK GVIP SSLF LQDD EDDD ELAG KSPE DLPL R
Gene ID:	7466
Uniprot:	O76024
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	100kDa
Observed MW:	110kDa

Synonyms:

WFS1, CTRCT41, WFRS, WFS, WFSL, wolframin

Background:

This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	WFS1: Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store. Highly expressed in heart followed by brain, placenta, lung and pancreas. Weakly expressed in liver, kidney and skeletal muscle. Also expressed in islet and beta-cell insulinoma cell line.
UniProt Protein Details:	Protein type:Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 4p16.1 Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; dendrite; integral to endoplasmic reticulum membrane Molecular Function:calmodulin binding; transcription activator binding; transporter activity; ubiquitin protein ligase binding; calcium-dependent protein binding; ATPase binding Biological Process: ER-associated protein catabolic process; protein maturation via protein folding; protein stabilization; olfactory behavior; positive regulation of proteolysis; unfolded protein response; negative regulation of transcription factor activity; glucose homeostasis; positive regulation of protein metabolic process; neurological system process; calcium ion homeostasis; endoplasmic reticulum calcium ion homeostasis; unfolded protein response, activation of signaling protein activity; sensory perception of sound; cellular protein metabolic process; visual perception; positive regulation of protein ubiquitination; negative regulation of programmed cell death; renal water homeostasis; negative regulation of neuron apoptosis; positive regulation of calcium ion transport; kidney development; positive regulation of growth; ER overload response Disease: Deafness, Autosomal Dominant 6; Wolfram Syndrome 1; Wolfram-like Syndrome, Autosomal Dominant; Cataract 41; Diabetes Mellitus, Noninsulin-dependent
NCBI Summary:	This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:	O76024
NCBI GenInfo Identifier:	317373289
NCBI Gene ID:	7466
NCBI Accession:	O76024.2
UniProt Secondary Accession:	O76024,Q8N6I3, Q9UNW6, B2R797, D3DVT1,
UniProt Related Accession:	O76024
Molecular Weight:	890
NCBI Full Name:	Wolframin
NCBI Synonym Full Names:	Wolfram syndrome 1 (wolframin)
NCBI Official Symbol:	WFS1
NCBI Official Synonym Symbols:	WFS; WFRS; WFSL; CTRCT41
NCBI Protein Information:	wolframin
UniProt Protein Name:	Wolframin
Protein Family:	Wolframin
UniProt Gene Name:	WFS1
UniProt Entry Name:	WFS1_HUMAN

	Western blot analysis of extracts of various cell lines, using WFS1 antibody (CAB1705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of HeLa cells using WFS1 Rabbit pAb (CAB1705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.