ChIP Antibodies
Anti-WEE1 Antibody (CAB16256)
- SKU:
- CAB16256
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-WEE1 Antibody |
| Antibody SKU: | CAB16256 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human WEE1 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | A synthetic peptide of human WEE1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 7465 |
| Uniprot: | P30291 |
| Cellular Location: | |
| Calculated MW: | 49kDa/71kDa |
| Observed MW: | 95kDa |
| Synonyms: | WEE1, WEE1A, WEE1hu, Wee1 |
| Background: | This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. |
| UniProt Protein Function: | Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. |
| UniProt Protein Details: | Protein type:EC 2.7.10.2; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Other group; WEE family Chromosomal Location of Human Ortholog: 11p15.4 Cellular Component: nucleolus; nucleoplasm; nucleus Molecular Function:kinase activity; protein binding; protein-tyrosine kinase activity Biological Process: G2/M transition of mitotic cell cycle; regulation of cell cycle |
| NCBI Summary: | This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30291 |
| NCBI GenInfo Identifier: | 1351419 |
| NCBI Gene ID: | 7465 |
| NCBI Accession: | P30291.2 |
| UniProt Secondary Accession: | P30291,B3KVE1, D3DQV0, |
| UniProt Related Accession: | P30291 |
| Molecular Weight: | 49,072 Da |
| NCBI Full Name: | Wee1-like protein kinase |
| NCBI Synonym Full Names: | WEE1 G2 checkpoint kinase |
| NCBI Official Symbol: | WEE1 |
| NCBI Official Synonym Symbols: | WEE1A; WEE1hu |
| NCBI Protein Information: | wee1-like protein kinase |
| UniProt Protein Name: | Wee1-like protein kinase |
| UniProt Synonym Protein Names: | Wee1A kinase |
| Protein Family: | Wee1-like protein kinase |
| UniProt Gene Name: | WEE1 |
| UniProt Entry Name: | WEE1_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using WEE1 antibody (CAB16256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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