Cell Biology Antibodies 3

Anti-WDR81 Antibody (CAB12780)

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SKU:
CAB12780
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-WDR81 Antibody
Antibody SKU:CAB12780
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 360-600 of human WDR81 (NP_689561.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 360-600 of human WDR81 (NP_689561.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RIGQ EMVQ QHLS EPVA TFFQ VFSQ LHEL RQQD LKLD PAGR GEGQ LPQV VFSD GQQR PVDP ALLD ELQK VFTL EMAY TIYV PFSC LLGD IIRK IIPN HELV GELA ALYL ESIS PSSR NPAS VEPT MPGT GPEW DPHG GGCP QDDG HSGT FGSV LVGN RIQI PNDS RPEN PGPL GPIS GVGG GGLG SGSD DNAL KQEL PRSV HGLS GNWL AYWQ YEIG VSQQ DAHF HFHQ IRLQ SFPG HSGA V
Gene ID:124997
Uniprot:Q562E7
Cellular Location:Mitochondrion
Calculated MW:74kDa/77kDa/79kDa/95kDa/197kDa/211kDa
Observed MW:260kDa
Synonyms:WDR81, CAMRQ2, PPP1R166, SORF-2
Background:This gene encodes a multi-domain transmembrane protein, which is predominantly expressed in the brain. Mutations in this gene are associated with autosomal recessive cerebellar ataxia, mental retardation, and dysequilibrium syndrome-2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:WDR81: 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 17p13.3

Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 2

NCBI Summary:This gene encodes a multi-domain transmembrane protein, which is predominantly expressed in the brain. Mutations in this gene are associated with autosomal recessive cerebellar ataxia, mental retardation, and dysequilibrium syndrome-2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
UniProt Code:Q562E7
NCBI GenInfo Identifier:403314383
NCBI Gene ID:124997
NCBI Accession:Q562E7.2
UniProt Secondary Accession:Q562E7,Q24JP6, Q8N277, Q8N3F3, Q8TEL1, B3KW16, B3KXU1 B7Z579, E9PHG7,
UniProt Related Accession:Q562E7
Molecular Weight:212kDa
NCBI Full Name:WD repeat-containing protein 81
NCBI Synonym Full Names:WD repeat domain 81
NCBI Official Symbol:WDR81  
NCBI Official Synonym Symbols:CAMRQ2; SORF-2; PPP1R166  
NCBI Protein Information:WD repeat-containing protein 81
UniProt Protein Name:WD repeat-containing protein 81
Protein Family:WD repeat-containing protein
UniProt Gene Name:WDR81  
Anti-WDR81 Antibody (CAB12780)Western blot analysis of extracts of Mouse brain, using WDR81 antibody (CAB12780) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-WDR81 Antibody (CAB12780)Immunofluorescence analysis of HeLa cells using WDR81 antibody (CAB12780) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-WDR81 Antibody (CAB12780)Immunofluorescence analysis of NIH/3T3 cells using WDR81 antibody (CAB12780) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-WDR81 Antibody (CAB12780)Immunofluorescence analysis of PC12 cells using WDR81 antibody (CAB12780) at dilution of 1:100. Blue: DAPI for nuclear staining.
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