Cell Biology Antibodies 12
Anti-WDR77 Monoclonal Antibody (CAB9921)
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- SKU:
- CAB9921
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- ICC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Mouse
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-WDR77 Monoclonal Antibody |
| Antibody SKU: | CAB9921 |
| Antibody Size: | 50uL, 100uL |
| Application: | WB ICC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Mouse |
| Immunogen: | Recombinant protein of human WDR77 |
| Application: | WB ICC |
| Recommended Dilution: | WB 1:500 - 1:2000 ICC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, HeLa, 293T, Jurkat, HepG2, MCF7, SW480 |
| Immunogen: | Recombinant protein of human WDR77 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 79084 |
| Uniprot: | Q9BQA1 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 29kDa/36kDa |
| Observed MW: | 39kDa |
| Synonyms: | WDR77, HKMT1069, MEP-50, MEP50, Nbla10071, p44, p44/Mep50 |
| Background: | The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | WDR77: a WD40 repeat protein. Component of the methylosome, a 20S complex containing at least ICLN, SKB1 and WDR77. Interacts with SKB1 and with SM proteins. The methylosome may regulate an early step in the assembly of U snRNPs, possibly the transfer of Sm proteins to the SMN-complex. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Adaptor/scaffold; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1p13.2 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; cytosol; nucleus Molecular Function:protein binding; ligand-dependent nuclear receptor transcription coactivator activity Biological Process: regulation of transcription from RNA polymerase II promoter; spliceosomal snRNP biogenesis; establishment and/or maintenance of chromatin architecture; positive regulation of cell proliferation; gene expression |
| NCBI Summary: | WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9BQA1 |
| NCBI GenInfo Identifier: | 32171507 |
| NCBI Gene ID: | 79084 |
| NCBI Accession: | Q9BQA1.1 |
| UniProt Secondary Accession: | Q9BQA1,Q3LID2, Q53FU2, Q6JZZ5, Q96GK4, Q9BWY3, B3KMW6 B4DP38, |
| UniProt Related Accession: | Q9BQA1 |
| Molecular Weight: | 342 |
| NCBI Full Name: | Methylosome protein 50 |
| NCBI Synonym Full Names: | WD repeat domain 77 |
| NCBI Official Symbol: | WDR77 |
| NCBI Official Synonym Symbols: | p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50 |
| NCBI Protein Information: | methylosome protein 50; androgen receptor cofactor p44; WD repeat-containing protein 77 |
| UniProt Protein Name: | Methylosome protein 50 |
| UniProt Synonym Protein Names: | Androgen receptor cofactor p44; WD repeat-containing protein 77; p44/Mep50 |
| Protein Family: | Methylosome protein |
| UniProt Gene Name: | WDR77 |
| UniProt Entry Name: | MEP50_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WDR77 antibody (CAB9921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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