Immunology Antibodies 2
Anti-WDR48 Antibody (CAB6854)
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- SKU:
- CAB6854
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-WDR48 Antibody |
| Antibody SKU: | CAB6854 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 450-677 of human WDR48 (NP_065890.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, A-431, SGC-7901, LO2, A-549, HT-1080, Mouse brain, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 450-677 of human WDR48 (NP_065890.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FSSP DGSD PKLN LGGL LLQA LLEY WPRT HVNP MDEE ENEV NHVN GEQE NRVQ KGNG YFQV PPHT PVIF GEAG GRTL FRLL CRDS GGET ESML LNET VPQW VIDI TVDK NMPK FNKI PFYL QPHA SSGA KTLK KDRL SASD MLQV RKVM EHVY EKII NLDN ESQT TSSS NNEK PGEQ EKEE DIAV LAEE KIEL LCQD QVLD PNMD LRTV KHFI WKSG GDLT LHYR QKST |
| Gene ID: | 57599 |
| Uniprot: | Q8TAF3 |
| Cellular Location: | Cytoplasm, Lysosome, Nucleus |
| Calculated MW: | 13kDa/45kDa/66kDa/75kDa/76kDa |
| Observed MW: | 76kDa |
| Synonyms: | WDR48, P80, SPG60, UAF1 |
| Background: | The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. |
| UniProt Protein Function: | WDR48: an ubiquitous lysosomal WD40 repeat protein. May play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Vesicle Chromosomal Location of Human Ortholog: 3p21.33 Cellular Component: intracellular membrane-bound organelle; lysosome; nucleus Molecular Function:protein binding Biological Process: skin development; protein deubiquitination; viral reproduction; skeletal morphogenesis; single fertilization; multicellular organism growth; homeostasis of number of cells; embryonic organ development; spermatogenesis; double-strand break repair via homologous recombination; positive regulation of epithelial cell proliferation |
| NCBI Summary: | The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. [provided by RefSeq, Sep 2016] |
| UniProt Code: | Q8TAF3 |
| NCBI GenInfo Identifier: | 74760390 |
| NCBI Gene ID: | 57599 |
| NCBI Accession: | Q8TAF3.1 |
| UniProt Related Accession: | Q8TAF3 |
| Molecular Weight: | 76kDa |
| NCBI Full Name: | WD repeat-containing protein 48 |
| NCBI Synonym Full Names: | WD repeat domain 48 |
| NCBI Official Symbol: | WDR48 |
| NCBI Official Synonym Symbols: | P80; UAF1; SPG60 |
| NCBI Protein Information: | WD repeat-containing protein 48 |
| UniProt Protein Name: | WD repeat-containing protein 48 |
| UniProt Synonym Protein Names: | USP1-associated factor 1; WD repeat endosomal protein; p80 |
| Protein Family: | WD repeat-containing protein |
| UniProt Gene Name: | WDR48 |
| UniProt Entry Name: | WDR48_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WDR48 antibody (CAB6854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry of paraffin-embedded rat liver using WDR48 antibody (CAB6854) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver using WDR48 antibody (CAB6854) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using WDR48 antibody (CAB6854) at dilution of 1:100 (40x lens). |
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