Epigenetics & Nuclear Signaling Antibodies 2

Anti-WDR4 Antibody (CAB16487)

(No reviews yet) Write a Review
SKU:
CAB16487
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-WDR4 Antibody
Antibody SKU:CAB16487
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-250 of human WDR4 (NP_061139.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:HeLa, HepG2, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-250 of human WDR4 (NP_061139.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SRFL ATSI ASSD DDSL FIYD CSAA EKKS QENK GEDA PLDQ GSGA ILAS TFSK SGSY FALT DDSK RLIL FRTK PWQC LSVR TVAR RCTA LTFI ASEE KVLV ADKS GDVY SFSV LEPH GCGR LELG HLSM LLDV AVSP DDRF ILTA DRDE KIRV SWAA APHS IESF CLGH TEFV SRIS VVPT QPGL LLSS SGDG TLRL WEYR SGRQ LHCC HLAS LQEL VDPQ APQK FAAS RIA
Gene ID:10785
Uniprot:P57081
Cellular Location:Nucleus
Calculated MW:29kDa/45kDa
Observed MW:45kDa
Synonyms:WDR4, TRM82, TRMT82
Background:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:WDR4: a WD40 repeat protein. Required for 7-methylguanosine modification of tRNA. Forms a complex with METTL1. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:protein binding; tRNA (guanine-N7-)-methyltransferase activity

Biological Process: tRNA modification

NCBI Summary:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:P57081
NCBI GenInfo Identifier:20141943
NCBI Gene ID:10785
NCBI Accession:P57081.2
UniProt Secondary Accession:P57081,Q9BVM5, Q9HCR3, A8KA58, B2RCA3, B4DNQ7, D3DSK3
UniProt Related Accession:P57081
Molecular Weight:29,946 Da
NCBI Full Name:tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4
NCBI Synonym Full Names:WD repeat domain 4
NCBI Official Symbol:WDR4  
NCBI Official Synonym Symbols:TRM82; TRMT82  
NCBI Protein Information:tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4
UniProt Protein Name:tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4
UniProt Synonym Protein Names:WD repeat-containing protein 4
Protein Family:tRNA
UniProt Gene Name:WDR4  
UniProt Entry Name:WDR4_HUMAN
Anti-WDR4 Antibody (CAB16487)Western blot analysis of extracts of various cell lines, using WDR4 antibody (CAB16487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
View AllClose

0 Reviews

View AllClose

Related Products