Cell Biology Antibodies 3

Anti-WDR35 Antibody (CAB12824)

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SKU:
CAB12824
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-WDR35 Antibody
Antibody SKU:CAB12824
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 871-1170 of human WDR35 (NP_065830.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:1000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 871-1170 of human WDR35 (NP_065830.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CSQP KAAV DTCV HLNQ WNKA VELA KNHS MKEI GSLL ARYA SHLL EKNK TLDA IELY RKAN YFFD AAKL MFKI ADEE AKKG SKPL RVKK LYVL SALL IEQY HEQM KNAQ RGKV KGKS SEAT SALA GLLE EEVL STTD RFTD NAWR GAEA YHFF ILAQ RQLY EGCV DTAL KTAL HLKD YEDI IPPV EIYS LLAL CACA SRAF GTCS KAFI KLKS LETL SSEQ KQQY EDLA LEIF TKHT SKDN RKPE LDSL MEGG EGKL PTCV ATGS PITE YQFW MCSV CKHG VLAQ EISH YSFC PLCH SPVG
Gene ID:57539
Uniprot:Q9P2L0
Cellular Location:Cytoplasm, centrosome, cilium axoneme, cilium basal body, cytoskeleton, microtubule organizing center
Calculated MW:132kDa/133kDa
Observed MW:133kDa
Synonyms:WDR35, CED2, IFT121, IFTA1, SRTD7
Background:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Two patients with Sensenbrenner syndrome / cranioectodermal dysplasia (CED) were identified with mutations in this gene, consistent with a possible ciliary function.
UniProt Protein Function:WDR35: a WD40 repeat protein. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: axoneme; centrosome; cilium

Molecular Function:phosphoinositide binding

Disease: Cranioectodermal Dysplasia 2; Short-rib Thoracic Dysplasia 7 With Or Without Polydactyly

NCBI Summary:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Two patients with Sensenbrenner syndrome / cranioectodermal dysplasia (CED) were identified with mutations in this gene, consistent with a possible ciliary function.[provided by RefSeq, Sep 2010]
UniProt Code:Q9P2L0
NCBI GenInfo Identifier:48474987
NCBI Gene ID:57539
NCBI Accession:Q9P2L0.3
UniProt Secondary Accession:Q9P2L0,Q4ZG01, Q8NE11, B3KVI5,
UniProt Related Accession:Q9P2L0
Molecular Weight:134kDa
NCBI Full Name:WD repeat-containing protein 35
NCBI Synonym Full Names:WD repeat domain 35
NCBI Official Symbol:WDR35  
NCBI Official Synonym Symbols:CED2; IFTA1; SRTD7; IFT121  
NCBI Protein Information:WD repeat-containing protein 35
UniProt Protein Name:WD repeat-containing protein 35
UniProt Synonym Protein Names:Intraflagellar transport protein 121 homolog
Protein Family:WD repeat-containing protein
UniProt Gene Name:WDR35  
Anti-WDR35 Antibody (CAB12824)Western blot analysis of extracts of LO2 cells, using WDR35 antibody (CAB12824) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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