Epigenetics & Nuclear Signaling Antibodies 4

Anti-WDR33 Antibody (CAB9531)

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SKU:
CAB9531
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-WDR33 Antibody
Antibody SKU:CAB9531
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human WDR33 (NP_001006623.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, U-87MG, MCF7, Mouse testis, Mouse thymus, Rat thymus, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human WDR33 (NP_001006623.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATE IGSP PRFF HMPR FQHQ APRQ LFYK RPDF AQQQ AMQQ LTFD GKRM RKAV NRKT IDYN PSVI KYLE NRIW QRDQ RDMR AIQP DAGY YNDL VPPI GMLN NPMN AVTT KFVR TSTN KVKC PVFV VRWT PEGR RLVT GASS GEFT LWNG LTFN FETI LQAH DSPV RAMT WSHN DMWM LTAD HGGY VKYW QSNM NNVK MFQA
Gene ID:55339
Uniprot:Q9C0J8
Cellular Location:Nucleus
Calculated MW:30kDa/38kDa/145kDa
Observed MW:170kDa
Synonyms:WDR33, NET14, WDC146
Background:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is highly expressed in testis and the protein is localized to the nucleus. This gene may play important roles in the mechanisms of cytodifferentiation and/or DNA recombination. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.
NCBI Summary:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is highly expressed in testis and the protein is localized to the nucleus. This gene may play important roles in the mechanisms of cytodifferentiation and/or DNA recombination. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9C0J8
NCBI GenInfo Identifier:55743157
NCBI Gene ID:55339
NCBI Accession:NP_001006623.1
UniProt Secondary Accession:Q9C0J8,Q05DP8, Q53FG9, Q587J1, Q69YF7, Q6NUQ0, Q9NUL1
UniProt Related Accession:Q9C0J8
Molecular Weight:146kDa
NCBI Full Name:pre-mRNA 3' end processing protein WDR33 isoform 2
NCBI Synonym Full Names:WD repeat domain 33
NCBI Official Symbol:WDR33  
NCBI Official Synonym Symbols:NET14; WDC146  
NCBI Protein Information:pre-mRNA 3' end processing protein WDR33
UniProt Protein Name:pre-mRNA 3' end processing protein WDR33
UniProt Synonym Protein Names:WD repeat-containing protein 33; WD repeat-containing protein WDC146
Protein Family:pre-mRNA 3' end processing protein
UniProt Gene Name:WDR33  
Anti-WDR33 Polyclonal Antibody (CAB9531)Western blot analysis of extracts of various cell lines, using WDR33 antibody (CAB9531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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