Cell Biology Antibodies 6

Anti-WDR18 Antibody (CAB15875)

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SKU:
CAB15875
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-WDR18 Antibody
Antibody SKU:CAB15875
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 243-432 of human WDR18 (NP_077005.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:A-549, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 243-432 of human WDR18 (NP_077005.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DLFT WPGQ RERS FHPE QDAG KVFK GHRN QVTC LSVS TDGS VLLS GSHD ETVR LWDV QSKQ CIRT VALK GPVT NAAI LLAP VSML SSDF RPSL PLPH FNKH LLGA EHGD EPRH GGLT LRLG LHQQ GSEP SYLD RTEQ LQAV LCST MEKS VLGG QDQL RVRV TELE DEVR NLRK INRD LFDF STRF ITRP AK
Gene ID:57418
Uniprot:Q9BV38
Cellular Location:Cytoplasm, Nucleus, nucleoplasm
Calculated MW:47kDa
Observed MW:47kDa
Synonyms:WDR18, Ipi3, R32184_1
Background:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation.
UniProt Protein Function:WDR18: a WD40 repeat protein. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm

Molecular Function:protein binding

Biological Process: rRNA processing

NCBI Summary:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BV38
NCBI GenInfo Identifier:37589023
NCBI Gene ID:57418
NCBI Accession:BC001648
UniProt Secondary Accession:Q9BV38,O60390, Q9BWR2,
UniProt Related Accession:Q9BV38
Molecular Weight:47,405 Da
NCBI Full Name:Homo sapiens WD repeat domain 18, mRNA
NCBI Synonym Full Names:WD repeat domain 18
NCBI Official Symbol:WDR18  
NCBI Official Synonym Symbols:Ipi3; R32184_1  
NCBI Protein Information:WD repeat-containing protein 18
UniProt Protein Name:WD repeat-containing protein 18
Protein Family:WD repeat-containing protein
UniProt Gene Name:WDR18  
UniProt Entry Name:WDR18_HUMAN
Anti-WDR18 Antibody (CAB15875)Western blot analysis of extracts of various cell lines, using WDR18 antibody (CAB15875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
Anti-WDR18 Antibody (CAB15875)Immunofluorescence analysis of U-2 OS cells using WDR18 antibody (CAB15875) at dilution of 1:100. Blue: DAPI for nuclear staining.
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