Cell Biology Antibodies 6

Anti-WDR12 Antibody (CAB15477)

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SKU:
CAB15477
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-WDR12 Antibody
Antibody SKU:CAB15477
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human WDR12 (NP_060726.3).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:A431, HeLa, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human WDR12 (NP_060726.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAQL QTRF YTDN KKYA VDDV PFSI PAAS EIAD LSNI INKL LKDK NEFH KHVE FDFL IKGQ FLRM PLDK HMEM ENIS SEEV VEIE YVEK YTAP QPEQ CMFH DDWI SSIK GAEE WILT GSYD KTSR IWSL EGKS IMTI VGHT DVVK DVAW VKKD SLSC LLLS ASMD QTIL LWEW NVER NKVK ALHC CRGH AGSV DSIA VDGS GTKF CSGS WDKM LKIW STVP TDEE DEME ESTN RPRK KQKT EQLG LTRT PIVT LSGH MEAV SSVL WSDA EEIC SASW DHTI
Gene ID:55759
Uniprot:Q9GZL7
Cellular Location:Nucleus, nucleolus, nucleoplasm
Calculated MW:47kDa
Observed MW:50kDa
Synonyms:WDR12, YTM1
Background:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein is highly similar to the mouse WD repeat domain 12 protein at the amino acid level. The protein encoded by this gene is a component of a nucleolar protein complex that affects maturation of the large ribosomal subunit.
UniProt Protein Function:WDR12: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Belongs to the WD repeat WDR12/YTM1 family.
UniProt Protein Details:

Protein type:Nucleolus

Chromosomal Location of Human Ortholog: 2q33.2

Cellular Component: nucleolus; nucleoplasm

Molecular Function:protein binding

Biological Process: cell proliferation; maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ribosomal large subunit biogenesis and assembly; rRNA processing

NCBI Summary:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein is highly similar to the mouse WD repeat domain 12 protein at the amino acid level. The protein encoded by this gene is a component of a nucleolar protein complex that affects maturation of the large ribosomal subunit.[provided by RefSeq, Dec 2008]
UniProt Code:Q9GZL7
NCBI GenInfo Identifier:20140802
NCBI Gene ID:55759
NCBI Accession:Q9GZL7.2
UniProt Secondary Accession:Q9GZL7,Q96HU0, Q9NV80, Q9NYI8, B3KPA9,
UniProt Related Accession:Q9GZL7
Molecular Weight:47,708 Da
NCBI Full Name:Ribosome biogenesis protein WDR12
NCBI Synonym Full Names:WD repeat domain 12
NCBI Official Symbol:WDR12  
NCBI Official Synonym Symbols:YTM1  
NCBI Protein Information:ribosome biogenesis protein WDR12
UniProt Protein Name:Ribosome biogenesis protein WDR12
UniProt Synonym Protein Names:WD repeat-containing protein 12
Protein Family:Ribosome biogenesis protein
UniProt Gene Name:WDR12  
UniProt Entry Name:WDR12_HUMAN
Anti-WDR12 Antibody (CAB15477)Western blot analysis of extracts of various cell lines, using WDR12 antibody (CAB15477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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