Cell Biology Antibodies 6
Anti-WDHD1 Antibody (CAB15396)
(No reviews yet)
Write a Review
- SKU:
- CAB15396
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-WDHD1 Antibody |
| Antibody SKU: | CAB15396 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 830-1129 of human WDHD1 (NP_009017.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | LO2, U-251MG, A-431, HeLa, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 830-1129 of human WDHD1 (NP_009017.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EEEE EEDF RKKL NAGY SNTA TEWS QPRF RNQV EEDA EDSG EADD EEKP EIHK PGQN SFSK STNS SDVS AKSG AVTF SSQG RVNP FKVS ASSK EPAM SMNS ARST NILD NMGK SSKK STAL SRTT NNEK SPII KPLI PKPK PKQA SAAS YFQK RNSQ TNKT EEVK EENL KNVL SETP AICP PQNT ENQR PKTG FQMW LEEN RSNI LSDN PDFS DEAD IIKE GMIR FRVL STEE RKVW ANKA KGET ASEG TEAK KRKR VVDE SDET ENQE EKAK ENLN LSKK QKPL DFST NQKL SAFA FKQE |
| Gene ID: | 11169 |
| Uniprot: | O75717 |
| Cellular Location: | Nucleus, nucleoplasm |
| Calculated MW: | 112kDa/125kDa |
| Observed MW: | 135kDa |
| Synonyms: | WDHD1, AND-1, AND1, CHTF4, CTF4 |
| Background: | The protein encoded by this gene contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms. |
| UniProt Protein Function: | WDHD1: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 14q22.2 Cellular Component: nucleoplasm; cytoplasm; chromosome, pericentric region Molecular Function:protein binding; DNA binding; RNA binding; chromatin binding Biological Process: RNA processing; regulation of chromosome organization and biogenesis |
| NCBI Summary: | The protein encoded by this gene contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75717 |
| NCBI GenInfo Identifier: | 5901892 |
| NCBI Gene ID: | 11169 |
| NCBI Accession: | NP_009017.1 |
| UniProt Related Accession: | O75717 |
| Molecular Weight: | 135kDa |
| NCBI Full Name: | WD repeat and HMG-box DNA-binding protein 1 isoform 1 |
| NCBI Synonym Full Names: | WD repeat and HMG-box DNA binding protein 1 |
| NCBI Official Symbol: | WDHD1 |
| NCBI Official Synonym Symbols: | AND1; CTF4; AND-1; CHTF4 |
| NCBI Protein Information: | WD repeat and HMG-box DNA-binding protein 1 |
| UniProt Protein Name: | WD repeat and HMG-box DNA-binding protein 1 |
| UniProt Synonym Protein Names: | Acidic nucleoplasmic DNA-binding protein 1; And-1 |
| Protein Family: | WD repeat and HMG-box DNA-binding protein |
| UniProt Gene Name: | WDHD1 |
| UniProt Entry Name: | WDHD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WDHD1 antibody (CAB15396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded Human esophageal cancer using WDHD1 Rabbit pAb (CAB15396) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using WDHD1 antibody (CAB15396) at dilution of 1:100. Blue: DAPI for nuclear staining. |
View AllClose
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
