Antibody Name:	Anti-WASF3 Antibody
Antibody SKU:	CAB16274
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human WASF3 (NP_001278894.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human WASF3 (NP_001278894.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RTRK EEWE RRKM GIEF MSDA KKLE QAGS AKED RVPS GSHA SDVT DYSY PATP NHSL HPQP VTPS YAAG DVPP HGPA SQAA EHEY RPPS ASAR HMAL NRPQ Q
Gene ID:	10810
Uniprot:	Q9UPY6
Cellular Location:
Calculated MW:	55kDa
Observed MW:	55kDa

Synonyms:

WASF3, Brush-1, SCAR3, WAVE3

Background:

This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	WASF3: Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Binds actin and the Arp2/3 complex. Expressed in ovary and brain. Belongs to the SCAR/WAVE family.
UniProt Protein Details:	Protein type:Actin-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 13q12 Cellular Component: cytoskeleton; lamellipodium; cytoplasm Molecular Function:actin binding Biological Process: positive regulation of myelination; lamellipodium biogenesis; regulation of cell shape; actin filament polymerization; cytoskeleton organization and biogenesis; protein complex assembly; oligodendrocyte development
NCBI Summary:	This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants [provided by RefSeq, May 2014]
UniProt Code:	Q9UPY6
NCBI GenInfo Identifier:	13699803
NCBI Gene ID:	10810
NCBI Accession:	NP_006637.2
UniProt Secondary Accession:	Q9UPY6,O94974, Q86VQ2,
UniProt Related Accession:	Q9UPY6
Molecular Weight:	55,293 Da
NCBI Full Name:	wiskott-Aldrich syndrome protein family member 3 isoform 1
NCBI Synonym Full Names:	WAS protein family, member 3
NCBI Official Symbol:	WASF3
NCBI Official Synonym Symbols:	SCAR3; WAVE3; Brush-1
NCBI Protein Information:	wiskott-Aldrich syndrome protein family member 3; protein WAVE-3; WASP family Verprolin-homologous protein 3; verprolin homology domain-containing protein 3
UniProt Protein Name:	Wiskott-Aldrich syndrome protein family member 3
UniProt Synonym Protein Names:	Protein WAVE-3; Verprolin homology domain-containing protein 3
Protein Family:	Wiskott-Aldrich syndrome protein family
UniProt Gene Name:	WASF3
UniProt Entry Name:	WASF3_HUMAN

	Western blot analysis of extracts of various cell lines, using WASF3 antibody (CAB16274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded human lung using WASF3 antibody (CAB16274) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using WASF3 antibody (CAB16274) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using WASF3 antibody (CAB16274) at dilution of 1:100 (40x lens).