Signal Transduction Antibodies 1

Anti-WASF1 Antibody (CAB14624)

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SKU:
CAB14624
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-WASF1 Antibody
Antibody SKU:CAB14624
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human WASF1 (NP_001020107.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, DU145, HeLa, K-562, 293T
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human WASF1 (NP_001020107.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VARA APVC ETVP VHPL PQGE VQGL PPPP PPPP LPPP GIRP SSPV TVTA LAHP PSGL HPTP STAP GPHV PLMP PSPP SQVI PASE PKRH PSTL PVIS DARS VLLE AIRK GIQL RKVE EQRE QEAK HERI ENDV ATIL SRRI AVEY SDSE DDSE FDEV DWLE
Gene ID:8936
Uniprot:Q92558
Cellular Location:Cell junction, Cytoplasm, cytoskeleton, focal adhesion, synapse
Calculated MW:61kDa
Observed MW:62kDa
Synonyms:WASF1, SCAR1, WAVE, WAVE1
Background:The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:WAVE1: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Belongs to the SCAR/WAVE family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: actin cytoskeleton; cytoskeleton; focal adhesion; lamellipodium; mitochondrial outer membrane; synapse

Molecular Function:actin binding; protein binding; protein complex binding; Rac GTPase binding

Biological Process: actin filament polymerization; cell motility; protein complex assembly; Rac protein signal transduction

NCBI Summary:The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q92558
NCBI GenInfo Identifier:2495730
NCBI Gene ID:8936
NCBI Accession:Q92558.1
UniProt Secondary Accession:Q92558,Q5SZK7, E1P5F2,
UniProt Related Accession:Q92558
Molecular Weight:61,652 Da
NCBI Full Name:Wiskott-Aldrich syndrome protein family member 1
NCBI Synonym Full Names:WAS protein family member 1
NCBI Official Symbol:WASF1  
NCBI Official Synonym Symbols:WAVE; SCAR1; WAVE1  
NCBI Protein Information:wiskott-Aldrich syndrome protein family member 1
UniProt Protein Name:Wiskott-Aldrich syndrome protein family member 1
UniProt Synonym Protein Names:Protein WAVE-1; Verprolin homology domain-containing protein 1
Protein Family:Wiskott-Aldrich syndrome protein family
UniProt Gene Name:WASF1  
UniProt Entry Name:WASF1_HUMAN
Anti-WASF1 Antibody (CAB14624)Western blot analysis of extracts of various cell lines, using WASF1 antibody (CAB14624) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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