Cell Biology Antibodies 1

Anti-WAS Antibody (CAB0978)

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SKU:
CAB0978
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-WAS Antibody
Antibody SKU:CAB0978
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WAS (NP_000368.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human
Positive Samples:HL-60, THP-1, Ramos
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WAS (NP_000368.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GAEH WTKE HCGA VCFV KDNP QKSY FIRL YGLQ AGRL LWEQ ELYS QLVY STPT PFFH TFAG DDCQ AGLN FADE DEAQ AFRA LVQE KIQK RNQR QSGD RRQL PPPP TPAN EERR GGLP PLPL HPGG DQGG PPVG PLSL GLAT VDIQ NPDI TSSR YRGL PAPG PSPA DKKR SGKK KISK ADIG APSG FKHV SHV
Gene ID:7454
Uniprot:P42768
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:52kDa
Observed MW:62kDa
Synonyms:WAS, IMD2, SCNX, THC, THC1, WASP, WASPA
Background: The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.
UniProt Protein Function:WASP: a member of the Wiskott-Aldrich syndrome (WAS) family of proteins. A cytoplasmic protein expressed exclusively in hematopoietic cells. Transduces signals from surface receptors to the actin cytoskeleton. Associates with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Mutated in Wiskott-Aldrich syndrome, a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xp11.4-p11.21

Cellular Component: intercellular junction; vesicle membrane; cytosol; actin cytoskeleton

Molecular Function:identical protein binding; protein binding; phospholipase binding; actin binding; SH3 domain binding; protein kinase binding

Biological Process: epidermis development; T cell activation; actin filament polymerization; regulation of catalytic activity; actin filament-based movement; actin polymerization and/or depolymerization; innate immune response; defense response; protein complex assembly; immune response; endosome transport; blood coagulation; T cell receptor signaling pathway

Disease: Thrombocytopenia 1; Neutropenia, Severe Congenital, X-linked

NCBI Summary:The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008]
UniProt Code:P42768
NCBI GenInfo Identifier:1722836
NCBI Gene ID:7454
NCBI Accession:P42768.4
UniProt Secondary Accession:P42768,Q9BU11, Q9UNJ9,
UniProt Related Accession:P42768
Molecular Weight:502
NCBI Full Name:Wiskott-Aldrich syndrome protein
NCBI Synonym Full Names:Wiskott-Aldrich syndrome
NCBI Official Symbol:WAS  
NCBI Official Synonym Symbols:THC; IMD2; SCNX; THC1; WASP  
NCBI Protein Information:wiskott-Aldrich syndrome protein; eczema-thrombocytopenia; thrombocytopenia 1 (X-linked)
UniProt Protein Name:Wiskott-Aldrich syndrome protein
Protein Family:Waspkinin
UniProt Gene Name:WAS  
UniProt Entry Name:WASP_HUMAN
Anti-WAS Antibody (CAB0978)Western blot analysis of extracts of various cell lines, using WAS Antibody (CAB0978) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Anti-WAS Antibody (CAB0978)Immunofluorescence analysis of U2OS cells using WAS antibody (CAB0978). Blue: DAPI for nuclear staining.
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