Name: Anti-VTI1A Antibody (CAB5162)
Brand: Cell Biology Antibodies 9
SKU: CAB5162
Availability: OutOfStock

Antibody Name:	Anti-VTI1A Antibody
Antibody SKU:	CAB5162
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human VTI1A (NP_660207.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HT-1080, Mouse lung, Mouse kidney, Mouse brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human VTI1A (NP_660207.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSD FEGY EQDF AVLT AEIT SKIA RVPR LPPD EKKQ MVAN VEKQ LEEA KELL EQMD LEVR EIPP QSRG MYSN RMRS YKQE MGKL ETDF KRSR IAYS DEVR NELL GDDG NSSE NQRA HLLD NTER LERS SRRL EAGY QIAV ETEQ IGQE MLEN LSHD REKI QRAR ERLR ETDA NLGK SSRI
Gene ID:	143187
Uniprot:	Q96AJ9
Cellular Location:	Cytoplasmic vesicle, Golgi apparatus membrane, Single-pass type IV membrane protein
Calculated MW:	23kDa/25kDa
Observed MW:	30kDa

Synonyms:

VTI1A, MMDS3, MVti1, VTI1RP2, Vti1-rp2

Background:

The protein encoded by this gene is a member of the family of soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptors (SNAREs) that function in intracellular trafficking. This family member is involved in vesicular transport between endosomes and the trans-Golgi network. It is a vesicle-associated SNARE (v-SNARE) that interacts with target membrane SNAREs (t-SNAREs). Polymorphisms in this gene have been associated with binocular function, and also with susceptibility to colorectal and lung cancers. A recurrent rearrangement has been found between this gene and the transcription factor 7-like 2 (TCF7L2) gene in colorectal cancers. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	VTI1A: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non- conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence. Belongs to the VTI1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 10q25.2 Cellular Component: autophagic vacuole; cell soma; clathrin-coated vesicle; endosome; Golgi apparatus; Golgi membrane; integral to membrane; perinuclear region of cytoplasm; SNARE complex; synaptic vesicle Molecular Function:protein binding; SNAP receptor activity; SNARE binding Biological Process: autophagy; ER to Golgi vesicle-mediated transport; intracellular protein transport; retrograde transport, endosome to Golgi; vesicle fusion with Golgi apparatus; voluntary musculoskeletal movement Disease: Multiple Mitochondrial Dysfunctions Syndrome 3
NCBI Summary:	The protein encoded by this gene is a member of the family of soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptors (SNAREs) that function in intracellular trafficking. This family member is involved in vesicular transport between endosomes and the trans-Golgi network. It is a vesicle-associated SNARE (v-SNARE) that interacts with target membrane SNAREs (t-SNAREs). Polymorphisms in this gene have been associated with binocular function, and also with susceptibility to colorectal and lung cancers. A recurrent rearrangement has been found between this gene and the transcription factor 7-like 2 (TCF7L2) gene in colorectal cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
UniProt Code:	Q96AJ9
NCBI GenInfo Identifier:	374095459
NCBI Gene ID:	143187
NCBI Accession:	Q96AJ9.2
UniProt Secondary Accession:	Q96AJ9,Q5W0D7, A2A307, B4E137,
UniProt Related Accession:	Q96AJ9
Molecular Weight:	23,435 Da
NCBI Full Name:	Vesicle transport through interaction with t-SNAREs homolog 1A
NCBI Synonym Full Names:	vesicle transport through interaction with t-SNAREs 1A
NCBI Official Symbol:	VTI1A
NCBI Official Synonym Symbols:	MMDS3; MVti1; VTI1RP2; Vti1-rp2
NCBI Protein Information:	vesicle transport through interaction with t-SNAREs homolog 1A
UniProt Protein Name:	Vesicle transport through interaction with t-SNAREs homolog 1A
UniProt Synonym Protein Names:	Vesicle transport v-SNARE protein Vti1-like 2; Vti1-rp2
Protein Family:	Vesicle transport through interaction with t-SNAREs
UniProt Gene Name:	VTI1A
UniProt Entry Name:	VTI1A_HUMAN

Western blot analysis of extracts of various cell lines, using VTI1A antibody (CAB5162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

VTI1A Antibody (PACO03520)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)