Cell Biology Antibodies 10
Anti-VSNL1 Antibody (CAB6999)
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- SKU:
- CAB6999
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-VSNL1 Antibody |
| Antibody SKU: | CAB6999 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human VSNL1 (NP_003376.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, 5637, Mouse brain, Mouse lung, Rat lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human VSNL1 (NP_003376.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGKQ NSKL APEV MEDL VKST EFNE HELK QWYK GFLK DCPS GRLN LEEF QQLY VKFF PYGD ASKF AQHA FRTF DKNG DGTI DFRE FICA LSIT SRGS FEQK LNWA FNMY DLDG DGKI TRVE MLEI IEAI YKMV GTVI MMKM NEDG LTPE QRVD KIFS KMDK NKDD QITL DEFK EAAK SDPS IVLL LQCD IQK |
| Gene ID: | 7447 |
| Uniprot: | P62760 |
| Cellular Location: | |
| Calculated MW: | 22kDa |
| Observed MW: | 23kDa |
| Synonyms: | VSNL1, HLP3, HPCAL3, HUVISL1, VILIP, VILIP-1 |
| Background: | This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. |
| UniProt Protein Function: | VSNL1: Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. Belongs to the recoverin family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 2p24.3 Molecular Function:calcium ion binding Biological Process: calcium-mediated signaling |
| NCBI Summary: | This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62760 |
| NCBI GenInfo Identifier: | 51338696 |
| NCBI Gene ID: | 7447 |
| NCBI Accession: | P62760.2 |
| UniProt Secondary Accession: | P62760,P28677, P29103, P42323, Q9UM20, D6W515, |
| UniProt Related Accession: | P62760 |
| Molecular Weight: | 191 |
| NCBI Full Name: | Visinin-like protein 1 |
| NCBI Synonym Full Names: | visinin-like 1 |
| NCBI Official Symbol: | VSNL1 |
| NCBI Official Synonym Symbols: | HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1 |
| NCBI Protein Information: | visinin-like protein 1; VLP-1; hippocalcin-like protein 3 |
| UniProt Protein Name: | Visinin-like protein 1 |
| UniProt Synonym Protein Names: | Hippocalcin-like protein 3; HLP3 |
| Protein Family: | Visinin-like protein |
| UniProt Gene Name: | VSNL1 |
| UniProt Entry Name: | VISL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VSNL1 Antibody (CAB6999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using VSNL1 antibody (CAB6999) at dilution of 1:200 (40x lens). |
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