Cell Biology Antibodies 4

Anti-VSIG1 Antibody (CAB13875)

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SKU:
CAB13875
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-VSIG1 Antibody
Antibody SKU:CAB13875
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-230 of human VSIG1 (NP_872413.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:BxP-C3, SGC-7901, OVCAR3, Mouse testis, Mouse stomach, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-230 of human VSIG1 (NP_872413.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ASRE QLSI QWSF FHKK EMEP ISIY FSQG GQAV AIGQ FKDR ITGS NDPG NASI TISH MQPA DSGI YICD VNNP PDFL GQNQ GILN VSVL VKPS KPLC SVQG RPET GHTI SLSC LSAL GTPS PVYY WHKL EGRD IVPV KENF NPTT GILV IGNL TNFE QGYY QCTA INRL GNSS CEID LTSS H
Gene ID:340547
Uniprot:Q86XK7
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:41kDa/45kDa
Observed MW:65kDa
Synonyms:VSIG1, 1700062D20Rik, GPA34, dJ889N15.1
Background:This gene encodes a member of the junctional adhesion molecule (JAM) family. The encoded protein contains multiple glycosylation sites at the N-terminal region, and multiple phosphorylation sites and glutamic acid/proline (EP) repeats at the C-terminal region. The gene is expressed in normal stomach and testis, as well as in gastric, esophageal and ovarian cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:VSIG1: 2 isoforms of the human protein are produced by alternative splicing
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq22.3

NCBI Summary:This gene encodes a member of the junctional adhesion molecule (JAM) family. The encoded protein contains multiple glycosylation sites at the N-terminal region, and multiple phosphorylation sites and glutamic acid/proline (EP) repeats at the C-terminal region. The gene is expressed in normal stomach and testis, as well as in gastric, esophageal and ovarian cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]
UniProt Code:Q86XK7
NCBI GenInfo Identifier:74759503
NCBI Gene ID:340547
NCBI Accession:Q86XK7.1
UniProt Secondary Accession:Q86XK7,Q6MZS4, C9J4P2,
UniProt Related Accession:Q86XK7
Molecular Weight:45,807 Da
NCBI Full Name:V-set and immunoglobulin domain-containing protein 1
NCBI Synonym Full Names:V-set and immunoglobulin domain containing 1
NCBI Official Symbol:VSIG1  
NCBI Official Synonym Symbols:GPA34; dJ889N15.1; 1700062D20Rik  
NCBI Protein Information:V-set and immunoglobulin domain-containing protein 1
UniProt Protein Name:V-set and immunoglobulin domain-containing protein 1
UniProt Synonym Protein Names:Cell surface A33 antigen; Glycoprotein A34
Protein Family:V-set and immunoglobulin domain-containing protein
UniProt Gene Name:VSIG1  
UniProt Entry Name:VSIG1_HUMAN
Anti-VSIG1 Antibody (CAB13875)Western blot analysis of extracts of various cell lines, using VSIG1 antibody (CAB13875) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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