Immunology Antibodies 1

Anti-VRK2 Antibody (CAB12243)

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SKU:
CAB12243
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-VRK2 Antibody
Antibody SKU:CAB12243
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 269-508 of human VRK2 (NP_006287.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 269-508 of human VRK2 (NP_006287.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DELP QSVL KWAP SGSS CCEI AQFL VCAH SLAY DEKP NYQA LKKI LNPH GIPL GPLD FSTK GQSI NVHT PNSQ KVDS QKAA TKQV NKAH NRLI EKKV HSER SAES CATW KVQK EEKL IGLM NNEA AQES TRRR QKYQ ESQE PLNE VNSF PQKI SYTQ FPNS FYEP HQDF TSPD IFKK SRSP SWYK YTST VSTG ITDL ESST GLWP TISQ FTLS EETN ADVY YYRI IIPV LLML VFLA LFFL
Gene ID:7444
Uniprot:Q86Y07
Cellular Location:Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion membrane, Nucleus, Single-pass type IV membrane protein
Calculated MW:44kDa/45kDa/55kDa/58kDa
Observed MW:54kDa
Synonyms:VRK2
Background:This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. The encoded protein acts as an effector of signaling pathways that regulate apoptosis and tumor cell growth. Variants in this gene have been associated with schizophrenia. Alternative splicing results in multiple transcript variants that differ in their subcellular localization and biological activity.
UniProt Protein Function:Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.
NCBI Summary:This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. The encoded protein acts as an effector of signaling pathways that regulate apoptosis and tumor cell growth. Variants in this gene have been associated with schizophrenia. Alternative splicing results in multiple transcript variants that differ in their subcellular localization and biological activity. [provided by RefSeq, Jan 2014]
UniProt Code:Q86Y07
NCBI GenInfo Identifier:90116515
NCBI Gene ID:7444
NCBI Accession:Q86Y07.3
UniProt Secondary Accession:Q86Y07,Q49AK9, Q53EU9, Q53S39, Q53S77, Q53TU1, Q86Y08 Q86Y09, Q86Y10, B4DKL0, D6W5D4, D6W5D6,
UniProt Related Accession:Q86Y07
Molecular Weight:58kDa
NCBI Full Name:Serine/threonine-protein kinase VRK2
NCBI Synonym Full Names:vaccinia related kinase 2
NCBI Official Symbol:VRK2  
NCBI Protein Information:serine/threonine-protein kinase VRK2
UniProt Protein Name:Serine/threonine-protein kinase VRK2
UniProt Synonym Protein Names:Vaccinia-related kinase 2
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:VRK2  
Anti-VRK2 Antibody (CAB12243)Western blot analysis of extracts of LO2 cells, using VRK2 antibody (CAB12243) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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