Antibody Name:	Anti-VPS4A Antibody
Antibody SKU:	CAB7096
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human VPS4A (NP_037377.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HeLa, U-87MG, SW620, A673, Mouse brain, Mouse testis, Mouse skeletal muscle, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human VPS4A (NP_037377.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTTS TLQK AIDL VTKA TEED KAKN YEEA LRLY QHAV EYFL HAIK YEAH SDKA KESI RAKC VQYL DRAE KLKD YLRS KEKH GKKP VKEN QSEG KGSD SDSE GDNP EKKK LQEQ LMGA VVME
Gene ID:	27183
Uniprot:	Q9UN37
Cellular Location:	Late endosome membrane, Midbody, Peripheral membrane protein, Prevacuolar compartment membrane
Calculated MW:	48kDa
Observed MW:	49kDa

Synonyms:

VPS4A, SKD1, SKD1A, SKD2, VPS4, VPS4-1

Background:

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18.

UniProt Protein Function:	VPS4A: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis. Belongs to the AAA ATPase family.
UniProt Protein Details:	Protein type:EC 3.6.4.6; Vesicle Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: spindle pole; centrosome; late endosome membrane; perinuclear region of cytoplasm; cytoplasm; midbody; cytosol; nucleus Molecular Function:protein C-terminus binding; protein domain specific binding; protein binding; ATPase activity, coupled; microtubule-severing ATPase activity; ATP binding Biological Process: ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cell separation during cytokinesis; viral reproduction; cytokinesis; viral infectious cycle; endosome transport; mitotic metaphase plate congression; negative regulation of cytokinesis; cytoplasmic microtubule organization and biogenesis; vesicle-mediated transport; protein transport; abscission; nuclear organization and biogenesis; vacuole organization and biogenesis
NCBI Summary:	The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UN37
NCBI GenInfo Identifier:	62511240
NCBI Gene ID:	27183
NCBI Accession:	Q9UN37.1
UniProt Secondary Accession:	Q9UN37,Q793F9, Q8VEJ9,
UniProt Related Accession:	Q9UN37
Molecular Weight:	437
NCBI Full Name:	Vacuolar protein sorting-associated protein 4A
NCBI Synonym Full Names:	vacuolar protein sorting 4 homolog A (S. cerevisiae)
NCBI Official Symbol:	VPS4A
NCBI Official Synonym Symbols:	SKD1; SKD2; VPS4; SKD1A; VPS4-1
NCBI Protein Information:	vacuolar protein sorting-associated protein 4A; VPS4A; hVPS4; SKD1-homolog; vacuolar sorting protein 4; vacuolar protein sorting factor 4A
UniProt Protein Name:	Vacuolar protein sorting-associated protein 4A
UniProt Synonym Protein Names:	Protein SKD2; VPS4-1; hVPS4
Protein Family:	Vacuolar protein sorting-associated protein
UniProt Gene Name:	VPS4A
UniProt Entry Name:	VPS4A_HUMAN

	Western blot analysis of extracts of various cell lines, using VPS4A antibody (CAB7096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using VPS4A antibody (CAB7096) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung using VPS4A antibody (CAB7096) at dilution of 1:100 (40x lens).