Cell Biology Antibodies 5

Anti-VPS36 Antibody (CAB15157)

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SKU:
CAB15157
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-VPS36 Antibody
Antibody SKU:CAB15157
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 70-220 of human VPS36 (NP_057159.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Rat liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 70-220 of human VPS36 (NP_057159.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EEQA AGIG KSAK IVVH LHPA PPNK EPGP FQSS KNSY IKLS FKEH GQIE FYRR LSEE MTQR RWEN MPVS QSLQ TNRG PQPG RIRA VGIV GIER KLEE KRKE TDKN ISEA FEDL SKLM IKAK EMVE LSKS IANK IKDK QGDI TEDE TIRF KSY
Gene ID:51028
Uniprot:Q86VN1
Cellular Location:Cytoplasm, Endosome, Late endosome, Membrane, Nucleus
Calculated MW:36kDa/43kDa
Observed MW:43kDa
Synonyms:VPS36, C13orf9, CGI-145, EAP45
Background:This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II.
UniProt Code:Q86VN1
NCBI GenInfo Identifier:71051598
NCBI Gene ID:51028
NCBI Accession:NP_057159
UniProt Secondary Accession:Q86VN1,Q3ZCV7, Q5H9S1, Q5VXB6, Q9H8Z5, Q9Y3E3, A8K125
UniProt Related Accession:Q86VN1
Molecular Weight:36,958 Da
NCBI Full Name:vacuolar protein-sorting-associated protein 36 isoform 1
NCBI Synonym Full Names:vacuolar protein sorting 36 homolog (S. cerevisiae)
NCBI Official Symbol:VPS36  
NCBI Official Synonym Symbols:EAP45; C13orf9; CGI-145  
NCBI Protein Information:vacuolar protein-sorting-associated protein 36; ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36
UniProt Protein Name:Vacuolar protein-sorting-associated protein 36
UniProt Synonym Protein Names:ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36
Protein Family:Vacuolar protein sorting-associated protein
UniProt Gene Name:VPS36  
UniProt Entry Name:VPS36_HUMAN
Anti-VPS36 Antibody (CAB15157)Western blot analysis of extracts of various cell lines, using VPS36 antibody (CAB15157) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-VPS36 Antibody (CAB15157)Immunofluorescence analysis of NIH-3T3 cells using VPS36 Polyclonal Antibody (CAB15157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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