Name: Anti-VPS35 Antibody (CAB7117)
Brand: Immunology Antibodies 2
SKU: CAB7117
Availability: OutOfStock

Antibody Name:	Anti-VPS35 Antibody
Antibody SKU:	CAB7117
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35 (NP_060676.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	293T, K-562, Mouse liver, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35 (NP_060676.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPTT QQSP QDEQ EKLL DEAI QAVK VQSF QMKR CLDK NKLM DALK HASN MLGE LRTS MLSP KSYY ELYM AISD ELHY LEVY LTDE FAKG RKVA DLYE LVQY AGNI IPRL YLLI TVGV VYVK SFPQ SRKD ILKD LVEM CRGV QHPL RGLF LRNY LLQC TRNI LPDE GEPT DEET TGDI SDSM DFVL LNFA EMNK LWVR MQHQ GHSR DREK RERE RQEL RILV GTNL VRLS QLEG VNVE RYKQ IVLT GILE QV
Gene ID:	55737
Uniprot:	Q96QK1
Cellular Location:	Cytoplasm, Early endosome, Endosome, Late endosome, Membrane, Peripheral membrane protein
Calculated MW:	91kDa
Observed MW:	92kDa

Synonyms:

VPS35, MEM3, PARK17

Background:

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

UniProt Protein Function:	VPS35: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Defects in VPS35 are the cause of Parkinson disease type 17 (PARK17). PARK17 is an autosomal dominant, adult- onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. Belongs to the VPS35 family.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 16q12 Cellular Component: retromer complex; lysosomal membrane; early endosome; late endosome; cytosol; endosome Molecular Function:protein binding; protein transporter activity Biological Process: intracellular protein transport; transcytosis; retrograde transport, endosome to Golgi Disease: Parkinson Disease 17
NCBI Summary:	This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96QK1
NCBI GenInfo Identifier:	25453321
NCBI Gene ID:	55737
NCBI Accession:	Q96QK1.2
UniProt Secondary Accession:	Q96QK1,Q561W2, Q9H016, Q9H096, Q9H4P3, Q9H8J0, Q9NRS7 Q9NVG2, Q9NX80, Q9NZK2,
UniProt Related Accession:	Q96QK1
Molecular Weight:	92kDa
NCBI Full Name:	Vacuolar protein sorting-associated protein 35
NCBI Synonym Full Names:	vacuolar protein sorting 35 homolog (S. cerevisiae)
NCBI Official Symbol:	VPS35
NCBI Official Synonym Symbols:	MEM3; PARK17
NCBI Protein Information:	vacuolar protein sorting-associated protein 35; hVPS35; maternal-embryonic 3
UniProt Protein Name:	Vacuolar protein sorting-associated protein 35
UniProt Synonym Protein Names:	Maternal-embryonic 3; Vesicle protein sorting 35
Protein Family:	Vacuolar protein sorting-associated protein
UniProt Gene Name:	VPS35
UniProt Entry Name:	VPS35_HUMAN

Western blot analysis of extracts of various cell lines, using VPS35 antibody (CAB7117) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.

Antibodies

Anti-VPS35 Antibody (CAB9278)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)