Cell Biology Antibodies 12

Anti-VPS33B Antibody (CAB8799)

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SKU:
CAB8799
Product Type:
Antibody
Applications:
WB
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-VPS33B Antibody
Antibody SKU:CAB8799
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 348-617 of human VPS33B (NP_061138.3).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:A-549, MCF7, DU145
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 348-617 of human VPS33B (NP_061138.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CESI MKKK TKQD FQEL IKTE HALL EGFN IRES TSYI EEHI DRQV SPIE SLRL MCLL SITE NGLI PKDY RSLK TQYL QSYG PEHL LTFS NLRR AGLL TEQA PGDT LTAV ESKV SKLV TDKA AGKI TDAF SSLA KRSN FRAI SKKL NLIP RVDG EYDL KVPR DMAY VFGG AYVP LSCR IIEQ VLER RSWQ GLDE VVRL LNCS DFAF TDMT KEDK ASSE SLRL ILVV FLGG CTFS EISA LRFL GREK GYRF IFLT TAVT NSAR LMEA MSEV KA
Gene ID:26276
Uniprot:Q9H267
Cellular Location:Cytoplasmic side, Cytoplasmic vesicle, Early endosome, Late endosome membrane, Lysosome membrane, Peripheral membrane protein, Recycling endosome, clathrin-coated vesicle
Calculated MW:60kDa/70kDa
Observed MW:62kDa
Synonyms:VPS33B
Background:Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants.
UniProt Code:Q9H267
NCBI GenInfo Identifier:119829191
NCBI Gene ID:
NCBI Accession:NP_061138
Molecular Weight:60kDa
NCBI Full Name:vacuolar protein sorting-associated protein 33B isoform 1
UniProt Protein Name:Vacuolar protein sorting-associated protein 33B
Protein Family:Vacuolar protein sorting-associated protein
UniProt Gene Name:VPS33B  
UniProt Entry Name:VP33B_HUMAN
Anti-VPS33B Polyclonal Antibody (CAB8799)Western blot analysis of extracts of various cell lines, using VPS33B antibody (CAB8799) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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