Antibody Name:	Anti-VPS26A Antibody
Antibody SKU:	CAB14265
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 208-327 of human VPS26A (NP_004887.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HT-29, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 208-327 of human VPS26A (NP_004887.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ELQL IKKE ITGI GPST TTET ETIA KYEI MDGA PVKG ESIP IRLF LAGY DPTP TMRD VNKK FSVR YFLN LVLV DEED RRYF KQQE IILW RKAP EKLR KQRT NFHQ RFES PESQ ASAE QPEM
Gene ID:	9559
Uniprot:	O75436
Cellular Location:	Cytoplasm, Early endosome, Endosome membrane, Peripheral membrane protein
Calculated MW:	28kDa/38kDa
Observed MW:	38kDa

Synonyms:

VPS26A, HB58, Hbeta58, PEP8A, VPS26

Background:

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	VPS26A: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Belongs to the VPS26 family.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 10q21.1 Cellular Component: cytosol; early endosome; endosome; endosome membrane; intracellular membrane-bound organelle; retromer complex; vesicle Molecular Function:protein binding; protein transporter activity Biological Process: intracellular protein transport; regulation of macroautophagy; retrograde transport, endosome to Golgi; Wnt receptor signaling pathway
NCBI Summary:	This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O75436
NCBI GenInfo Identifier:	25453454
NCBI Gene ID:	9559
NCBI Accession:	O75436.2
UniProt Secondary Accession:	O75436,Q8TBH4, Q9H982, A8MZ56, B2RDD3,
UniProt Related Accession:	O75436
Molecular Weight:	28,938 Da
NCBI Full Name:	Vacuolar protein sorting-associated protein 26A
NCBI Synonym Full Names:	VPS26, retromer complex component A
NCBI Official Symbol:	VPS26A
NCBI Official Synonym Symbols:	HB58; PEP8A; VPS26; Hbeta58
NCBI Protein Information:	vacuolar protein sorting-associated protein 26A
UniProt Protein Name:	Vacuolar protein sorting-associated protein 26A
UniProt Synonym Protein Names:	Vesicle protein sorting 26A; hVPS26
Protein Family:	Vacuolar protein sorting-associated protein
UniProt Gene Name:	VPS26A
UniProt Entry Name:	VP26A_HUMAN

	Western blot analysis of extracts of various cell lines, using VPS26A antibody (CAB14265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of NIH-3T3 cells using VPS26A Polyclonal Antibody (CAB14265) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.