Name: Anti-VPS25 Antibody (CAB13132)
Brand: Cell Biology Antibodies 3
SKU: CAB13132
Availability: OutOfStock

Antibody Name:	Anti-VPS25 Antibody
Antibody SKU:	CAB13132
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-176 of human VPS25 (NP_115729.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BxPC-3, Mouse brain, Mouse heart, Rat liver, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-176 of human VPS25 (NP_115729.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAMS FEWP WQYR FPPF FTLQ PNVD TRQK QLAA WCSL VLSF CRLH KQSS MTVM EAQE SPLF NNVK LQRK LPVE SIQI VLEE LRKK GNLE WLDK SKSS FLIM WRRP EEWG KLIY QWVS RSGQ NNSV FTLY ELTN GEDT EDEE FHGL DEAT LLRA LQAL QQEH KAEI ITVS DGRG VKFF
Gene ID:	84313
Uniprot:	Q9BRG1
Cellular Location:	Cytoplasm, Endosome membrane, Nucleus, nucleoplasm
Calculated MW:	20kDa
Observed MW:	20kDa

Synonyms:	VPS25, DERP9, EAP20, FAP20
Background:	This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1.

UniProt Protein Function:	VPS25: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. Belongs to the VPS25 family.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 17q21.31 Cellular Component: nucleoplasm; mitochondrion; nucleolus; endosome membrane; nucleus; cytosol Molecular Function:protein binding; protein homodimerization activity; structural molecule activity Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; endosome transport
NCBI Summary:	This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]
UniProt Code:	Q9BRG1
NCBI GenInfo Identifier:	73920459
NCBI Gene ID:	84313
NCBI Accession:	Q9BRG1.1
UniProt Related Accession:	Q9BRG1
Molecular Weight:	20kDa
NCBI Full Name:	Vacuolar protein-sorting-associated protein 25
NCBI Synonym Full Names:	vacuolar protein sorting 25 homolog
NCBI Official Symbol:	VPS25
NCBI Official Synonym Symbols:	DERP9; EAP20; FAP20
NCBI Protein Information:	vacuolar protein-sorting-associated protein 25
UniProt Protein Name:	Vacuolar protein-sorting-associated protein 25
UniProt Synonym Protein Names:	Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25
Protein Family:	Vacuolar protein sorting-associated protein
UniProt Gene Name:	VPS25
UniProt Entry Name:	VPS25_HUMAN

	Western blot analysis of extracts of various cell lines, using VPS25 antibody (CAB13132) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using VPS25 Polyclonal Antibody (CAB13132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using VPS25 Polyclonal Antibody (CAB13132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.