Cell Death Antibodies 1
Anti-VPS24 Antibody (CAB10306)
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- SKU:
- CAB10306
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-VPS24 Antibody |
| Antibody SKU: | CAB10306 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human VPS24 (NP_057163.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, LO2, 293T, SW480 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human VPS24 (NP_057163.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGLF GKTQ EKPP KELV NEWS LKIR KEMR VVDR QIRD IQRE EEKV KRSV KDAA KKGQ KDVC IVLA KEMI RSRK AVSK LYAS KAHM NSVL MGMK NQLA VLRV AGSL QKST EVMK AMQS LVKI PEIQ ATMR ELSK EMMK AGII EEML EDTF ESMD DQEE MEEE AEME IDRI LFEI TAGA LGKA PSKV TDAL PEPE PPGA MAAS EDEE EEEE ALEA MQSR LATL RS |
| Gene ID: | 51652 |
| Uniprot: | Q9Y3E7 |
| Cellular Location: | Cytoplasm, Endosome, Late endosome membrane, Lipid-anchor, Membrane, cytosol |
| Calculated MW: | 17kDa/20kDa/25kDa/28kDa |
| Observed MW: | 23kDa |
| Synonyms: | CHMP3, CGI-149, NEDF, VPS24 |
| Background: | This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. |
| UniProt Protein Function: | VPS24: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress- mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells. Belongs to the SNF7 family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: cytoplasmic vesicle; cytosol; intracellular; late endosome; plasma membrane Molecular Function:phosphatidylcholine binding; protein binding; protein homodimerization activity Biological Process: cell separation during cytokinesis; endosome transport; macroautophagy; mitotic metaphase plate congression; nuclear organization and biogenesis; protein heterooligomerization; protein polymerization; regulation of viral reproduction; viral infectious cycle |
| NCBI Summary: | This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. [provided by RefSeq, Nov 2010] |
| UniProt Code: | Q9Y3E7 |
| NCBI GenInfo Identifier: | 73917763 |
| NCBI Gene ID: | 51652 |
| NCBI Accession: | Q9Y3E7.3 |
| UniProt Secondary Accession: | Q9Y3E7,Q3ZTS9, Q53S71, Q53SU5, Q9NZ51, A8K3W0, B4DG34 B8ZZM0, B8ZZX5, |
| UniProt Related Accession: | Q9Y3E7 |
| Molecular Weight: | 25kDa |
| NCBI Full Name: | Charged multivesicular body protein 3 |
| NCBI Synonym Full Names: | charged multivesicular body protein 3 |
| NCBI Official Symbol: | CHMP3 |
| NCBI Official Synonym Symbols: | NEDF; VPS24; CGI-149 |
| NCBI Protein Information: | charged multivesicular body protein 3 |
| UniProt Protein Name: | Charged multivesicular body protein 3 |
| UniProt Synonym Protein Names: | Chromatin-modifying protein 3; Neuroendocrine differentiation factor; Vacuolar protein sorting-associated protein 24; hVps24 |
| Protein Family: | Vacuolar protein sorting-associated protein |
| UniProt Gene Name: | CHMP3 |
 | Western blot analysis of extracts of various cell lines, using VPS24 antibody (CAB10306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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