Signal Transduction Antibodies 2
Anti-VNN1 Antibody (CAB3347)
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- SKU:
- CAB3347
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-VNN1 Antibody |
| Antibody SKU: | CAB3347 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-415 of human VNN1 (NP_004657.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, A-431, HepG2, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-415 of human VNN1 (NP_004657.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LLSQ LDSH PSHS AVVN WTSY ASSI EALS SGNK EFKG TVFF DEFT FVKL TGVA GNYT VCQK DLCC HLSY KMSE NIPN EVYA LGAF DGLH TVEG RYYL QICT LLKC KTTN LNTC GDSA |
| Gene ID: | 8876 |
| Uniprot: | O95497 |
| Cellular Location: | Cell membrane, GPI-anchor, Lipid-anchor |
| Calculated MW: | 57kDa |
| Observed MW: | 60kDa |
| Synonyms: | VNN1, HDLCQ8, Tiff66, vanin 1 |
| Background: | This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. |
| UniProt Protein Function: | VNN1: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. Belongs to the CN hydrolase family. BTD/VNN subfamily. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; EC 3.5.1.92; Hydrolase; Membrane protein, integral; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cell adhesion; Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 6q23-q24 Cellular Component: integral to membrane; plasma membrane Molecular Function:pantetheine hydrolase activity Biological Process: positive regulation of T cell differentiation in the thymus; chronic inflammatory response; cell-cell adhesion; pantothenate metabolic process; innate immune response; response to oxidative stress; cell motility; inflammatory response; acute inflammatory response |
| NCBI Summary: | This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. [provided by RefSeq, Feb 2009] |
| UniProt Code: | O95497 |
| NCBI GenInfo Identifier: | 76803830 |
| NCBI Gene ID: | 8876 |
| NCBI Accession: | O95497.2 |
| UniProt Related Accession: | O95497 |
| Molecular Weight: | |
| NCBI Full Name: | Pantetheinase |
| NCBI Synonym Full Names: | vanin 1 |
| NCBI Official Symbol: | VNN1 |
| NCBI Official Synonym Symbols: | HDLCQ8; Tiff66 |
| NCBI Protein Information: | pantetheinase |
| UniProt Protein Name: | Pantetheinase |
| UniProt Synonym Protein Names: | Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1; Vanin-1 |
| Protein Family: | Pantetheinase |
| UniProt Gene Name: | VNN1 |
| UniProt Entry Name: | VNN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VNN1 antibody (CAB3347) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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